| 摘要: |
| 目的分析microRNA-155（miR-155）在系统性红斑狼疮（SLE）患者外周血B细胞中的表达及干预后B细胞表达IgG水平。方法收集66例SLE患者及10例健康人群外周血，分离出B细胞，采用实时荧光定量PCR（qRT-PCR）检测miR-155在B细胞中的表达水平，利用电转染技术使miRNA抑制剂干预SLE患者外周血B细胞miR-155的表达，然后以ELISA检测B细胞干预后培养液上清液IgG的水平。结果qRT-PCR结果显示，与健康人比较，SLE患者外周血B细胞中miR-155表达上调（P＜0.05），通过miRNA抑制剂下调SLE患者B细胞中的miR-155的表达后，B细胞分泌的IgG水平明显降低。结论SLE患者的B细胞中表达上调的miR-155可能参与了SLE患者B细胞功能调控。 |
| 关键词: 系统性红斑狼疮 微小 RNA 微小 RNA -155 B 细胞 IgG |
| DOI：10.12056/j.issn.1006-2785.2018.40.10.2017-1016 |
| 分类号: |
| 基金项目:宁波市科技局科研基金资助项目（2015A610179） |
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| Expression of miRNA-155 in peripheral B cells of patients with systemic lupus erythematosus |
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Ningbo Medical Treatment Center Lihuili Hospital
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| Abstract: |
| Objective To investigate the expression of microRNA-155 (miR-155) in peripheral B cells of the patients with systemic lupus erythematosus (SLE). Methods Sixty-six SLE patients and 10 healthy controls were enrolled in the study. The B cells were isolated from peripheral blood, and the expression of miR-155 in B cells was detected by fluorescence quantitative real-time RT-PCR (qRT-PCR). The levels of IgG in supernatants of B cells were determined by enzyme-linked immunosorbent assay (ELISA) after miR-155 expression was intervened by electrotransfection. Results The expression of miR-155 was significantly increased in B cells of the patients with SLE compared with healthy controls (P＜0.05). After intervention of the miR-155 expression with miRNA inhibitor, the levels of total IgG in the supernatants of the cultured B cells were significantly de- creased. Conclusion The study indicates that the up-regulated miR-155 expression may be involved in the regulation of B cell function in systemic lupus erythematosus. |
| Key words: Systemic lupus erythematosus microRNA miR-155 B cell IgG |
