| 摘要: |
| 目的建立测定小鼠肝组织腺苷酸含量的反相高效液相色谱（RP-HPLC）法，并观察溴苯腈中毒对小鼠肝组织能量代谢的影响。方法将24只SD大鼠随机分为急性中毒组予溴苯腈（97％溴苯腈原药以10％二甲亚砜为溶媒配成10mg/ml溶液)200mg/kg灌胃染毒和正常对照组（予10％二甲亚砜溶液20ml/kg灌胃)，每组12只。采用RP-HPLC法（C18ODS色谱柱，流动相为乙腈磷酸缓冲液，检测波长254nm，柱温16℃，流速1.1ml/min）测定小鼠肝组织三磷酸腺苷（ATP）、二磷酸腺苷（ADP）、磷酸腺苷（AMP）含量。结果APT、ADP和AMP浓度在1～200mg/L范围内线性关系良好；3者平均回收率分别为（93.63±8.02）%、（87.02±8.28）%、（94.99±6.97）%；日内峰面积变异系数分别为0.79%、1.02%和1.05%，日间峰面积变异系数分别为2.09%、1.75%和2.17%；溴苯腈中毒组ATP明显低于正常对照组，AMP明显高于正常对照组。结论该方法可简便、快速、准确测定小鼠肝组织ATP、ADP和AMP含量；溴苯腈对小鼠肝组织能量代谢有明显抑制作用。 |
| 关键词: 反相高效液相色谱法 肝 腺苷酸 溴苯腈 |
| DOI：10.12056/j.issn.1006-2785.2018.40.6.2017-1140 |
| 分类号: |
| 基金项目:浙江省自然科学基金项目（LY15H150008）；浙江省卫生厅资助项目（2014KYB153） |
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| Determination of adenosine content in liver tissue of mice with acute bromoxynil intoxication by RP-HPLC |
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the Second Affiliated Hospital of Wenzhou Medical University
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| Abstract: |
| Objective To develop a new RP-HPLC method for determination of adenosine content and to evaluate the effect of bromoxynil on liver energy metabolism in mice. Methods A C18ODS column was used in RP-HPLC with acetonitrile phosphate buffer as mobile phase, the detection wavelength was 254 nm, the column temperature was 16℃ and the flow rate was 1.1 ml/min. Adenosine (ATP, ADP and AMP) contents in the liver tissue of mice were measured. Results The average recovery rates of ATP, ADP and AMP were (93.63±8.02)%, (87.02 ±8.28)% and (94.99 ±6.97)%, respectively. The relative standard deviations (RSDs) of within - day were 0.79%, 1.02% and 1.05%; and the RSDs of between - days were 2.09%, 1.75% and 2.17%, respectively. Conclusion The RP-HPLC method is simple, rapid and sensitive in analysis of the adenosine contents in mouse liver. Bromoxynil can cause hepatic injury and disturb liver energy metabolism in mice. |
| Key words: RP-HPLC Liver Adenosine Bromoxynil |
