| 摘要: |
| 目的探讨苍南地区前列腺癌（PCa）与高危型人乳头瘤病毒（HPV）16/18感染的关系。方法选取2012至2016年PCa患者64例（PCa组）和同期良性前列腺增生（BPH）患者72例（BPH组）作对照研究。两组患者的存档石蜡标本采用原位杂交方法检测HPV16/18感染情况，免疫组化EnVision法检测p16表达情况；第二代杂交捕获法检测2016年患者尿液中高危型HPV，电镜观测部分患者活检新鲜组织标本。结果PCa组石蜡标本中HPV16/18和p16阳性率均明显高于BPH组，差异均有统计学意义（均P＜0.01）；两组尿液中高危型HPV阳性率比较差异有统计学意义（P＜0.05）。电镜下10例PCa新鲜组织标本中4例可见病毒样颗粒，7例BPH新鲜组织标本均未见病毒样颗粒。结论本地区PCa患者HPV16/18的感染率高于BPH患者，HPV16/18的感染可能是本地区PCa的发病因素之一。 |
| 关键词: 前列腺癌 良性前列腺增生 高危型人乳头瘤病毒 尿液 电镜 |
| DOI：10.12056/j.issn.1006-2785.2018.40.7.2017-1218 |
| 分类号: |
| 基金项目:温州市公益性社会发展（医疗卫生）科技项目（Y20160189） |
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| Association between HPV16/18 infection and prostate cancer in Cangnan |
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Cangnan Hospital of Wenzhou Medical University
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| Abstract: |
| Objective To investigate the association between high-risk human papilloma virus (HPV16/18) infection and prostate cancer (PCa) in Cangnan. Methods 64 PCa patients and 72 patients with benign prostatic hyperplasia (BPH) were enrolled from 2012 to 2016. HPV16/18 infection was detected by hybridization in situ in paraffin-embedded specimens, and the expression of p16 was detected by immunohistochemical En Vision method. High risk HPV testing was performed by hybridcapture method in urine samples collected from patients in 2016. The biopsy specimens were examined by electron
microscopy. Results The positive rates of HPV16/18 and expression p16 in PCa tissue were significantly higher than those in the BPH group (both P＜ 0.01).There was significant difference in the positive rate of high-risk HPV between the urine samples from two groups (P＜ 0.05). Virus-like particles were observed in 4 of 10 PCa biopsy specimens under electron microscopy, and no virus-like particles were found in 7 BPH specimens. Conclusion The results indicate that prostate cancer may be associated
with HPV16/18 infection in Cangnan. |
| Key words: Prostate cancer Benign prostatic hyperplasia High-risk human papillomavirus Urine Electron micro- scope |
