| 摘要: |
| 目的研究动脉粥样硬化患者含热蛋白结构域3的核苷酸结合寡聚化样受体（NLRP3）炎性体相关分子表达与疾病之间的关系。方法采用酶联免疫吸附（ELISA）法测定40例动脉粥样硬化患者（实验组）和40例健康志愿者（对照组）血浆中IL-1β、IL-18和IL-37的含量，采用定量聚合酶链反应（PCR）检测两组单核细胞来源的巨噬细胞（MDM）表达NLPR3、凋亡相关斑点样蛋白（ASC）和IL-1βmRNA水平，采用ELISA技术检测胆固醇结晶诱导MDM分泌IL-1β和TNF-琢的水平。结果动脉粥样硬化患者血浆中的IL-1β和IL-18含量均显著高于对照组，差异均有统计学意义（均P＜0.05）；IL-37高于对照组，但无统计学差异（P＞0.05）；动脉粥样硬化患者MDM中NLPR3、ASC和IL-1βmRNA表达均显著高于对照组，差异均有统计学意义（均P＜0.05）；且经胆固醇结晶刺激后IL-1β、TNF-琢的分泌水平显著高于对照组，差异均有统计学意义（均P＜0.05）。结论NLRP3炎性体活化可能参与动脉粥样硬化的炎症反应过程。 |
| 关键词: 白细胞介素 -1β 动脉粥样硬化 含热蛋白结构域 3 的核苷酸结合寡聚化样受体 炎性体 |
| DOI：10.12056/j.issn.1006-2785.2018.40.11.2017-1247 |
| 分类号: |
| 基金项目:浙江省自然科学基金项目（LY14H100003）；桐乡市科技局项目（201502037） |
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| Expression of NLRP3 inflammasome pathway molecules in patients with atherosclerosis |
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Tongxiang Second People's Hospital
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| Abstract: |
| Objective To investigate the expression of NLRP3 inflammasome related molecules in patients with atherosclerosis and its significance. Methods The plasma levels of IL-1β, IL-18 and IL-37 in 40 patients with atherosclerosis and 40 healthy controls were detected with ELISA. Expression of NLRP3, ASC and IL-1 β mRNA in monocytes derived macrophages (MDM) were detected by real time RT-PCR. The IL-1β and TNF- α levels in MDM after stimulation with cholesterol crystal were also analyzed with ELISA. Results IL-1β and IL-18 levels in atherosclerotic patients were significantly higher than those in normal controls (P ＜0.05), while there was no significant difference in IL-37 level between patients and controls (P ＞0.05). Expression of NLRP3, ASC and IL-1 β mRNA in MDM and IL-1 β、TNF- α secreted by MDM of atherosclerotic patients were significantly higher than those of controls (P ＜0.05). Conclusion The NLRP3 inflammasome pathway may play an important role in the patients with atherosclerosis. |
| Key words: Interleukin-1beta Atherosclerosis NLRP3 protein Inflammasomes, |
