| 摘要: |
| 目的探讨慢性失眠障碍患者血清丙二醛（MDA）和谷胱甘肽过氧化物酶（GSH-Px）水平及其影响因素。方法收集慢性失眠障碍患者110例（患者组）和睡眠正常的健康者90例（健康对照组），采用匹兹堡睡眠质量指数问卷（PSQI）对慢性失眠障碍患者的睡眠质量进行评定，采用汉密尔顿抑郁量表（HAMD-24）和汉密尔顿焦虑量表（HAMA-14）对慢性失眠障碍患者的焦虑、抑郁水平进行评定，受试者血清MDA水平采用比色法（TBA法）进行测定，血清GSH-PX水平采用酶联免疫吸附法（ELISA）进行测定。结果患者组患者的血清MDA水平明显高于健康对照组，血清GSH-PX水平明显低于健康对照组，差异均有统计学意义（均P＜0.05）。多元线性回归分析结果显示，患者组患者的PSQI总分为血清MDA水平的独立影响因素（标准化茁=0.354，P=0.008），另外患者组PSQI总分和BMI是血清GSH-PX水平的独立影响因素（标准化茁=-0.393，P=0.002；标准化茁=0.254，P=0.025）。结论慢性失眠障碍患者体内存在血清MDA、GSH-PX水平异常，氧化应激可能是慢性失眠障碍引起的病理生理变化之一。 |
| 关键词: 慢性失眠障碍 丙二醇 谷胱甘肽过氧化物酶 氧化应激 |
| DOI：10.12056/j.issn.1006-2785.2018.40.6.2017-1466 |
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| Serum malondialdehyde, glutathione peroxidase level and its related factors in chronic insomnia patients |
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the First Affiliated Hospital of Wenzhou Medical University
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| Abstract: |
| Objective To investigate the serum levels of malondialdehyde(MDA) and glutathione peroxidase (GSH-Px) in chronic insomnia patients and its related factors. Methods A total of 110 chronic insomnia patients and 90 healthy controls with normal sleep were enrolled in the study. We used the Pittsburgh Sleep Quality Index (PSQI) to assess the sleep quality of chronic insomnia patients. The 24-itme Hamilton Depression Scale (HAMD-24) and 14-item Hamilton Anxiety Scale (HAMA-24) were used to assess the levels of anxiety and depression. Serum MDA was measured by thiobarbituric acid (TBA) method, and serum GSH-PX was measured by enzyme-linked immunosorbent assay (ELISA). Results Chronic insomnia patients had significantly
lower GSH-Px levels and higher MDA levels, compared with the controls (all P ＜0.05). Multiple linear regression analysis
indicated that the PSQI score was significantly affected patients serum MDA level (茁=0.354, P=0.008), and the PSQI score and BMI were significantly affected patients serum GSH-PX level(茁=-0.393, P=0.002 and 茁=0.254, P=0.025). Conclusion The results indicate that the disturbance of serum MDA and GSH-PX level exists in chronic insomnia patients. Oxidative stress may be a pathophysiological progress induced by chronic insomnia. |
| Key words: Chronic insomnia Malondialdehyde Glutathione peroxidase Oxidative stress |
