| 摘要: |
| 目的探讨长链非编码RNA（LncRNA）浆细胞瘤转化迁移基因1（PVT1）在神经胶质瘤中的表达及临床意义。方法采用qRT-PCR方法检测并比较53例神经胶质瘤组织（观察组）及6例正常脑组织（对照组）中LncRNAPVT1的表达量。根据神经胶质瘤组织中LncRNAPVT1的中位表达量分为高表达组与低表达组，分析LncRNAPVT1表达量与临床病理因素的关系。采用Kaplan-Meier法绘制不同LncRNAPVT1表达量的神经胶质瘤患者生存曲线，并分析LncRNAPVT1表达量与神经胶质瘤患者预后的关系。结果观察组LncRNAPVT1的表达量明显高于对照组（P＜0.01)。其中高级别胶质瘤（WHOⅢ~Ⅳ级）的LncRNAPVT1表达量明显高于低级别胶质瘤（WHOⅠ~Ⅱ级）（P＜0.01）。LncRNAPVT1表达量与神经胶质瘤WHO分级密切相关（P＜0.01），与患者年龄、性别、Karnofsky功能状态评分（KPS）评分、肿瘤大小等均无关（均P＞0.05）。经log-rank检验，高表达组患者预后较低表达组差，差异有统计学意义（P＜0.01）。结论LncRNAPVT1在神经胶质瘤组织中高表达，表达量与神经胶质瘤WHO分级及患者预后密切相关；LncRNAPVT1可能成为判断神经胶质瘤恶性程度及预后的新型分子标记物。 |
| 关键词: 神经胶质瘤 长链非编码 RNA 浆细胞瘤转化迁移基因 1 预后 |
| DOI：10.12056/j.issn.1006-2785.2017.39.23.2017-1489 |
| 分类号: |
| 基金项目:浙江省中医药科技计划项目（2016ZQ004）；浙江省医药卫生科技计划科研基金项目（2017KY179） |
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| Expression of long non-coding RNA PVT1 in brain glioma and its clinical significance |
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Zhejiang Hospital
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| Abstract: |
| Objective To investigate the expression of long-coding RNA (lncRNA) of plasmacytoma variant translocation gene 1 (PVT1) in glioma and its clinical significance. Methods qRT-PCR was applied to detect the expression of lncRNA PVT1 in 53 specimens of glioma (glioma group) and 6 specimens of normal brain tissues (control group). The survival curves were generated with Kaplan-Meier method. The correlation between the expression of lncRNA PVT1 and clinicopathological features,
prognosis of patients was analyzed. Results The expression of lncRNA PVT1 was significantly up-regulated in glioma group compared with that the control group (P＜0.01). The expression of lncRNA PVT1 in high-grade neuroglioma (WHO III-IVgrade) was significantly higher than that in low-grade neuroglioma (WHO I-II grade) (P＜0.01). The expression of lncRNA PVT1 was significantly associated with the WHO grading of glioma (P＜0.01), but not with age, gender, Karnofsky Performance Status (KPS) score and tumor size (All P ＞0.05). Log-rank test revealed that patients with high expression of lncRNA PVT1 was significantly associated with worse prognosis compared with those with low expression(P＜0.01). Conclusion LncRNA PVT1 is up-regulated
in neuroglioma tissues. The expression of lncRNA PVT1 is closely associated with WHO grading system and prognosis of patients, indicating that lncRNA PVT1 may serve as a novel molecular biomarker for determination of the malignant degree and prognosis of neuroglioma. |
| Key words: Glioma Long non-coding RNA PVT1 Prognosis |
