| 摘要: |
| 目的探索人巨细胞病毒（HCMV）UL133基因型对婴幼儿肝功能损伤的分子机制。方法选择收治入院的68例HCMV肝炎患儿和66例同期门诊体检肝功能正常且确诊为HCMV感染的患儿为研究对象。其中UL133基因型筛查阳性的66例肝炎患儿为UL133阳性组，UL133基因型筛查阴性的64例体检患儿为UL133阴性组。检测并比较患儿尿液中HCMVUL133基因型，血液中Caspase-3、Caspase-6蛋白表达水平及肝功能指标。结果肝炎患儿尿液中UL133基因型筛查阳性率达94.1%，明显高于体检患儿的3.0%（P＜0.05）。UL133阳性组患儿血液中ALT、Caspase-3、Caspase-6蛋白表达水平均明显高于UL133阴性组（P＜0.05）。两组患儿血AST、TBiL、DBiL、谷氨酰转移酶（GGT）比较差异均无统计学意义（均P＞0.05）。结论携带有UL133基因型的HCMV感染婴幼儿后，可能通过诱导Caspase-3、Caspase-6蛋白表达上调，导致肝功能损伤。 |
| 关键词: 人巨细胞病毒 UL133 肝炎 基因 |
| DOI： |
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| 基金项目:温州市科技计划项目（Y20160018） |
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| Mechanisms of UL133 gene-induced liver function impairment in infants with HCMV infection |
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the Second Affiliated Hospital of Wenzhou Medical University
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| Abstract: |
| Objective To investigate the mechanisms of UL133 gene-induced liver function impairment ininfants with human cytomegalovirus (HCMV) infection. Methods Sixty-eight infants with HCMV hepatitis admitted in the Second Affiliated Hospital of Wenzhou Medical University and 66 healthy infants infected with HCMV were enrolled in the study.The HCMV UL133 gene expression in the urine was detected by RT-PCR and plasma levels of Caspase-3 and Caspase-6 were measured by ELISA. Results The positive rates of UL133 gene expression in infants with hepatits and healthy infants were 94.1%and 3.0%,
respectively (P＜0.05). The plasma levels of ALT, Caspase-3 and Caspase-6 in positive group were significantly higher than
those in negative group (P＜0.05), while there were no significant differences in TBiL, DBiL, GGT and AST levels between two groups (P ＞0.05). Conclusion The UL133 genotype is associated with liver function injury, which may be mediated by up-regulation of Caspase-3 and Caspase-6 in infants with HCMV infection. |
| Key words: HCMV UL133 Hepatitis Gene |
