| 摘要: |
| 目的分析蛋白磷酸酶2A的癌性抑制因子（CIP2A）在鼻咽癌组织中的表达情况，并探讨其与鼻咽癌临床病理特征的关系。方法选取鼻咽癌病理标本70例，另择正常鼻咽部组织30例作为对照。采用免疫组化法检测CIP2A蛋白表达情况，采用RT-PCR法检测CIP2AmRNA表达情况。观察并比较CIP2A蛋白、mRNA在鼻咽癌及鼻咽部正常组织中的表达情况；分析CIP2AmRNA表达水平与鼻咽癌临床病理特征的关系。结果鼻咽癌组织中CIP2A蛋白、mRNA阳性表达率均高于鼻咽部正常组织（65.71%vs26.67%、81.43%vs16.67%，均P＜0.05）。CIP2AmRNA表达水平与鼻咽癌患者性别、年龄、病理类型无关（均P＞0.05），而与表示肿瘤范围的T分期、淋巴结有无转移的N分期及综合的临床分期有关（均P＜0.05）。结论CIP2A与鼻咽癌的发生和浸润转移有关，可作为判断鼻咽癌恶性程度的一个重要生物学指标。 |
| 关键词: 鼻咽癌 CIP2A 免疫组化 RT-PCR |
| DOI：10.12056/j.issn.1006-2785.2017.39.19.2017-1670 |
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| Expression of CIP2A in nasopharyngeal carcinoma and its clinicopathological significance |
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Zhejiang Taizhou hospital
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| Abstract: |
| Objective To investigate the expression of cancerous inhibitor of protein phosphatase 2A (CIP2A) in nasopharyngeal carcinoma (NPC) and its clinicopathological significance. Methods The expression of CIP2A protein and mRNA in 70 specimens of NPC and 30 specimens of normal nasopharyngeal tissue was detected by immunohistochemistry and RT-PCR, respectively. The correlation between CIP2A expression and clinicopathological features of NPC was analyzed. Results The expression of CIP2A protein and mRNA was higher in NPC than that in normal nasopharyngeal tissue (65.71% vs 26.67%, 81.43% vs 16.67 % , respectively, both P＜0.05). The expression of CIP2A mRNA was correlated with tumor size, lymph node metastasis and clinic stages of NPC (all P ＜0.05), not with the gender, age of the patients and pathological type (P ＞0.05). Conclusion CIP2A may be associated with the occurrence, invasion and metastasis of NPC, suggesting that it can be used as an indicator for malignant degree of nasopharyngeal carcinoma. |
| Key words: Nasopharyngeal carcinoma(NPC) Cancerous inhibitor of protein phosphatase 2A (CIP2A) Immunohisto- chemistry RT-PCR |
