| 摘要: |
| 目的研究巨噬细胞迁移抑制因子（MIF）及炎症因子在昼夜节律改变小鼠心脏缺血再灌注损伤中的作用。方法36只小鼠在12h/12h昼夜交替和持续黑暗环境下饲养2周，分为Normal组、Normal缺血再灌注组、Disrupted组、Disrupted缺血再灌注组，每组9只，4周后完成造模，Normal组和Disrupted组行心超检测、心肌病理检查、心肌MIF、IL-6、TNF-α、IL-8检测。另对Normal缺血再灌注组及Disrupted缺血再灌注组行心脏缺血再灌注处理，处理后分别检测心肌IL-6、TNF-α、IL-8等炎症因子表达，心肌组织局部炎症浸润程度及超声心动图。结果Disrupted组小鼠心肌MIF表达较Normal组显著下降（P＜0.05）。Disrupted缺血再灌注组和Normal缺血再灌注组相比，心脏IL-6、TNF-α、IL-8表达显著增高，病理检查示心肌局部炎症浸润增加，心超示心功能受损加重（均P＜0.05）。结论改变小鼠昼夜节律致心肌MIF蛋白表达下降，心肌缺血再灌注后炎症因子IL-6、TNF-α、IL-8表达增加，局部炎症反应加重，心功能恶化。 |
| 关键词: 黑暗环境 昼夜节律 缺血再灌注损伤 MIF IL-6 TNF-α IL-8 |
| DOI：10.12056/j.issn.1006-2785.2018.40.11.2017-1872 |
| 分类号: |
| 基金项目:浙江省自然科学基金项目（LY13H020004）；温州市科技局项目（Y20130167，Y20130211） |
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| Down-regulated MIF aggravates cardiac ischemia / reperfusion injury in mice with circadian disturbance |
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Fourth Affiliated Hospital of Zhejiang University School of Medicine
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| Abstract: |
| Objective To examine the effect of macrophage migration inhibitory factor (MIF) and inflammatory factors on cardiac ischemia/reperfusion (I/R) injury in mice with disturbed circadian rhythm. Methods Thirty six mice were randomly housed in 12h light/dark cycle (normal) or constant darkness (disrupted) environment, and equally divided into normal group, normal I/R group, disrupted group and disrupted I/R group. After 4 weeks, the heart function, myocardial HE staining, myocardial MIF protein and the inflammatory cytokines were detected in normal group and disrupted group. The cardiac ischemia reperfusion injury was induced in normal I/R group and disrupted I/R group, respectively. Then the expression of inflammatory
cytokines s IL-6, TNF-α and IL-8, the degree of local inflammatory infiltration and the heart function were examined.
Results WB revealed that cardiac MIF expression was significantly lower in the disrupted group than that in the normal group. Following ischemia and reperfusion, mice in the disrupted I/R group had more serious cardiac inflammatory response and lower heart function index than the normal I/R group (P＜0.05); the cardiac expression of IL-6, TNF-α and IL-8 was higher than that of normal I/R group (P＜0.05). Conclusion Disturbing the circadian rhythm of mice by constant dark environment can lead to the
lower expression of MIF and higher expression of inflammatory factors after cardiac ischemia/reperfusion injury, indicating that down-regulated MIF may be associated with aggravation of local inflammatory and worse cardiac function. |
| Key words: Darkness environment Circadian rhythm Ischemia reperfusion injury MIF IL-6 TNF-α IL-8 |
