| 摘要: |
| 目的探讨大黄素对急性肝功能衰竭（ALF）大鼠肝脏的保护作用机制。方法将SD大鼠随机分为健康对照组（6只）、ALF模型组（24只）和大黄素保护组（24只）。模型组、保护组用D-氨基半乳糖（D-Gal）建立ALF动物实验模型，12h后保护组按10mg/kg腹腔注射大黄素，2次/d；健康对照组注射等量0.9%氯化钠溶液。模型组、保护组在建模后24、72、120、168h随机各取6只，取门静脉血检测肝功能指标，取肝组织检测其NF-资B水平，并进行比较分析。结果模型组造模后24、72、120hALT水平均明显高于对照组（均P＜0.05），24、72、120、168hAST水平均明显高于对照组（均P＜0.05）；保护组造模后24、72hALT、AST水平均明显高于对照组（均P＜0.05）；造模后72hALT、AST水平均为模型组高于保护组（均P＜0.05），造模后120、168hAST水平亦均为模型组高于保护组（均P＜0.05）。HE染色见对照组大鼠肝组织结构完整，能清晰看到肝索；模型组大鼠肝小叶结构明显被破坏，肝细胞大片坏死。造模后24、72、120h模型组、保护组NF-资B水平均明显高于对照组（均P＜0.05），两组均在72h达到高峰；模型组造模后168h明显高于对照组（P＜0.05）；造模后24、72、120、168h模型组均高于保护组（均P＜0.05）。结论大黄素对ALF大鼠的肝脏功能有保护作用，其机制可能与下调肝脏组织中NF-资B表达有关。 |
| 关键词: 急性肝功能衰竭 大黄素 核因子 -资B 大鼠 |
| DOI：10.12056/j.issn.1006-2785.2017.39.7.2017-21 |
| 分类号: |
| 基金项目:浙江省中医药科学研究基金计划项目（2010ZA118）；台州市椒江区科技计划项目（112070） |
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| Effects of emodin on liver function in rats with acute liver failure and its mechanism |
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YU Ying, WANG Fenglin
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Taizhou Municipal hospital
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| Abstract: |
| Objective To investigate the effect of emodin on liver function in acute hepatic failure and its mechanism. Methods SD rats were randomly divided into three groups: healthy control group (n=6), acute liver failure (ALF) model group (n=24) and emodin treatment group (n=24). Acute liver failure was induced by intraperitoneal injection of D-galactosamine (D-Gal). Twelve hours after the model was established, the emodin group received intraperitoneal injection of 10mg/kg emodin twice daily. At 24, 72, 120 and 168h after modeling, 6 rats were sacrificed in ALF group and emodin group. The portal vein blood samples were collected and hepatic function was examined. Liver tissue was fixed with 10% formalin, and the expression of NF-资B in liver tissue was detected by immunohistochemistry. Results After 24h of modeling, the levels of alanine aminotransferase(ALT) and aspartate aminotransferase(AST) in serum were significantly higher than those in healthy control group (P＜0.01), after 72h of modeling, the liver function of rats in ALF group was significantly higher than of emodin group at the same
time point (P＜0.01), after 24h of modeling, the expression of NF-资B in ALF group was significantly higher than that in healthy control group (P＜0.01), reached the peak at 72h, then decreased after 120h. The expression of NF-资B in emodin group was significantly lower than that in ALF group at 72,120 and 168h after modeling (P＜0.01). Conclusion Emodin has protective effect on acute liver failure in rats, which may be related to the down-regulation of NF-资B expression. |
| Key words: Acute liver failure Emodin NF-资B Rat |
