| 摘要: |
| 目的探讨磷酸化的真核翻译起始因子结合蛋白（p-4E-BP1）在结直肠癌组织中的表达及与临床病理特征、预后的关系。方法采用免疫组化染色检测164例患者结直肠癌组织中p-4E-BP1表达，采用qRT-PCR、Westernbolt法检测结直肠癌组织及其癌旁正常组织中4E-BP1、p-4E-BP1的mRNA、蛋白表达，应用Kaplan-Meier法和Cox比例风险回归模型对结直肠癌患者进行生存分析。结果164例结直肠癌患者中p-4E-BP1均有表达，其中低表达69例（42.1%），高表达95例（57.9%）。p-4E-BP1表达与结直肠癌患者的肿瘤分化程度、浸润深度、淋巴结转移及TNM分期均有关，其中细胞分化较差、浸润较深、淋巴结转移、TNM分期较晚的结直肠癌患者p-4E-BP1表达均较高（均P＜0.05）。结直肠癌组织中4E-BP1mRNA、蛋白表达与癌旁正常组织比较，差异均无统计学意义（均P＞0.05）；而p-4E-BP1蛋白表达较癌旁正常组织明显增高（P＜0.05）。p-4E-BP1高表达组无进展生存期、总生存期均明显短于低表达组（均P＜0.05）。p-4E-BP1蛋白表达是影响患者术后总生存期、无进展生存期的独立风险因素（RR=5.414、4.754，均P＜0.05）。结论p-4E-BP1高表达与肿瘤进展以及不良预后相关，p-4E-BP1可作为临床判断结直肠癌患者预后的一个新指标。 |
| 关键词: 结肠直肠癌 4E-BP1 磷酸化 临床病理特征 预后 |
| DOI：10.12056/j.issn.1006-2785.2017.39.23.2017-223 |
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| Expression of phosphorylated 4E-BP1 and its relationship to prognosis in colorectal cancer |
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the Second Affiliated Hospital of Wenzhou Medical University
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| Abstract: |
| Objective To evaluate the expression of phosphorylated eukaryotic translation initiation factor 4E-binding
protein (p-4E-BP1) and to assess its relationship with clinicopathological features and prognosis in patients with colorectal cancer. Methods The expression of p-4E-BP1 in 164 specimens of colorectal cancer tissue was detected with immunohistochemistry. The expression of 4E-BP1 mRNA and protein in colorectal cancer and adjacent normal tissues were detected with qRT-PCR and Western bolt, respectively. Kaplan-Meier method and Cox proportional hazards regression model were used to analyze the survival of patients with colorectal cancer. Results p-4E-BP1 was positive in all colorectal cancer samples, including 95 (57.9%) cases with high expression and 69 (42.1%) cases with low expression. The expression level of p-4E-BP1 was significantly associated with tumor differentiation, invasive depth, lymph node metastasis and TNM stage. High
expression of p-4E-BP1 was observed in colorectal cancer patients with poor differentiation, deeper infiltration, regional lymph node metastasis and later TNM stage (all P ＜0.05). Although there were no significant differences in mRNA and protein expression of 4E-BP1 between tumor tissue and adjacent normal colorectal tissue, the phosphorylation level of 4E-BP1 was markedly increased in colorectal cancer tissue (P＜0.05). Kaplan-Meier survival analysis showed that the overall survival and progression-free survival of the p-4E-BP1 high expression group was significantly shorter than these of the low expression group(both P＜0.05). Cox proportional hazards model revealed that p-4E-BP1 was an independent adverse prognostic factor for both overall survival (RR=5.414, P ＜0.05) and progression-free survival (RR=4.754, P ＜0.05) in colorectal cancer patients. Conclusion High p-4E-BP1 expression is associated with tumor progression and adverse prognosis. p-4E-BP1 might serve as
a novel biomarker to predict the clinical outcome of patients with colorectal cancer. |
| Key words: Colorectal cancer 4E-BP1 Phosphorylation Clinicopathological features Prognosis |
