| 摘要: |
| 目的筛选及验证浸润性肺腺癌组织上调表达基因，寻找肺腺癌诊断和预后评估的新型分子标记物。方法收集经手术病理证实的浸润性肺腺癌患者6例,制作癌组织及相应癌旁组织基因表达谱芯片,进一步通过Geneontology、Pathway分析、标记物预测分析和基因网络关系分析方法对芯片数据进行处理，结合TheLungCancer数据库检索与肺癌发生相关的mRNA信息，筛选出浸润性肺腺癌组织上调表达基因。另收集经手术病理证实的浸润性肺腺癌患者97例，抽提癌组织及相应癌旁组织RNA，逆转录，设计芯片筛选出的上调表达目的基因引物，实时荧光定量PCR技术（qPCR）扩增目的基因，以PUM1为内参基因，获得各样本组织ΔCt值，与癌旁组织对比，分析103例癌组织目的基因表达情况。结果基因芯片数据结合数据库，初步筛选出浸润性肺腺癌组织14个上调表达基因，分别为SPINK1、ITGA11、TOX3、SPP1、MCM10、MMP12、COL11A1、SIX1、FOXA1、CLIC6、TMEM45B、GDF15、CEACAM1、ESR1。qPCR验证结果表明，与相应癌旁组织相比，SPINK1mRNA、TOX3mRNA、MMP12mRNA、CLIC6mRNA、TMEM45BmRNA在浸润性肺腺癌组织上调表达，差异均有统计学意义（P＜0.05或0.01），而ITGA11mRNA、SPP1mRNA、MCM10mRNA、COL11A1mRNA、SIX1mRNA、FOXA1mRNA、GDF15mRNA、CEACAM1mRNA、ESR1mRNA在肺腺癌组织表达未见上调，差异均无统计学意义（均P＞0.05）。结论本研究成功筛选及验证SPINK1、TOX3、MMP12、CLIC6、TMEM45BmRNA水平在浸润性肺腺癌组织上调表达，提示可作为肺腺癌诊断和预后评估的新型分子标记物。 |
| 关键词: 浸润性肺腺癌 基因芯片 差异表达基因 分子标记物 |
| DOI：10.12056/j.issn.1006-2785.2018.40.3.2017-418 |
| 分类号: |
| 基金项目:浙江省科技厅分析测试科技计划项目（2015C37024）；浙江省医药卫生科技计划一般项目（2015KYB411)；舟山市公益类科技项目（2014C31065） |
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| Screening and verification of up-regulated genes in invasive lung adenocarcinoma |
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Affiliated Zhoushan Hospital of Wenzhou Medical University
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| Abstract: |
| Objective To screen and verify the up-regulated genes in invasive lung adenocarcinoma. Methods Tissue samples from 6 cases of invasive lung adenocarcinoma confirmed by surgery and pathology were collected. Gene expression profiling chips for cancer tissue and adjacent tissue were made. The chips data were analyzed with Gene Ontology, Pathway Analysis, Marker Analysis, Gene Network Relationship Analysis. Up-regulated genes in invasive lung adenocarcinoma were screened, with the data base of The Lung Cancer and mRNA information related to lung cancer. The expression of up-regulated genes in cancer and pericancerous tissue samples of 97 patients with invasive lung adenocarcinoma were detected by RT-PCR and compared between cancer and pericancerous tissues. Results Fourteen up-regulated expression genes in invasive lung adenocarcinoma were screened by the microarray and the database. The up-regulated expression genes were SPINK1, ITGA11, TOX3, SPP1, MCM10, MMP12, COL11A1, SIX1, FOXA1, CLIC6, TMEM45B, GDF15, CEACAM1 and ESR1. Compared with
paracancerous tissues, the mRNA expressions of SPINK1, TOX3, MMP12, CLIC6 and TMEM45B were up-regulated in cancer tissues(P＜0.01or 0.05). There was no significant difference in the mRNA expressions of ITGA11, SPP1, MCM10, COL11A1, SIX1, FOXA1, TMEM45B, GDF15, CEACAM1 and ESR1 between two groups(P ＞0.05). Conclusion The expressions of SPINK1, TOX3, MMP12, CLIC6 and TMEM45B mRNA are up-regulated in invasive lung adenocarcinoma, the value of these gene need to be
further explored. |
| Key words: Invasive lung adenocarcinoma Gene microarray Differentially expressed genes Molecular markers |
