| 摘要: |
| 目的观察青蒿琥酯（ART）对胃癌细胞株BGC-823的裸鼠移植瘤生长的影响，并探讨其可能的作用机制。方法将胃癌BGC-823细胞注射至裸鼠右侧背部近腋下部位皮下，建立荷瘤裸鼠模型并随机分为空白对照组（0mg/kgART组）、60mg/kgART组、120mg/kgART组、240mg/kgART组，每组5只。各组裸鼠每天腹腔注射ART1次（空白对照组注射0.9%氯化钠溶液），连续15d，观察裸鼠移植瘤生长情况，并采用RT-PCR、免疫组化及Westernblot法测定血管内皮生长因子（VEGF）及钙中性蛋白酶2（Calpain2）表达情况。结果4组裸鼠移植瘤体积比较有统计学差异（P＜0.05），两两比较亦有统计学差异（均P＜0.05），与空白对照组比较，60mg/kgART组、120mg/kgART组、240mg/kgART组裸鼠移植瘤体积均明显缩小，ART表现出剂量依赖性的抑瘤作用。4组胃癌BGC-823细胞VEGF、Calpain2mRNA与蛋白表达水平比较均有统计学差异（均P＜0.05），两两比较亦有统计学差异（均P＜0.05），与空白对照组比较，60mg/kgART组、120mg/kgART组、240mg/kgART组细胞VEGFmRNA与蛋白相对表达水平均下降，Calpain2mRNA与蛋白相对表达水平均升高，且VEGF相对表达水平随ART浓度升高而降低，而Calpain2相对表达水平随ART浓度升高而升高。结论ART在胃癌移植瘤模型体内起剂量依赖性的抑瘤作用，该作用机制可能与下调VEGF的表达、上调Calpain2的表达有关。 |
| 关键词: 青蒿琥酯 胃癌 裸鼠模型 |
| DOI：10.12056/j.issn.1006-2785.2017.39.14.2017-802 |
| 分类号: |
| 基金项目:温州市科技计划项目（Y20140105、Y20140111） |
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| Effects of artesunate on transplanted human gastric cancer BGC-823 in nude mice |
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WANG Weiming, ZHOU Xiang, ZHENG Zhiqiang
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the First Affiliated Hospital of Wenzhou Medical University
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| Abstract: |
| Objective To investigate the effect of artesunate on transplanted human gastric cancer in nude mice. Methods Human gastric cancer BGC-823 cells were inoculated in nude mice. The tumor-bearing nude mice were treated with artesunate at a dose of 60, 120, or 240 mg/ (kg•d), i.p. for 15 d. The changes of body weights and tumor size were observed. The
expression of VEGF and Calpain2 mRNA and proteins were detected by RT-PCR, immunohistochemical and Western Blot, respectively. Results Artesunate suppressed BGC-823 tumor growth in nude mice in a dose-dependent manner. The expression of VEGF mRNA and protein were decreased and the expression of Calpain2 mRNA and protein were increased in
cancer cells of artesunate-treated nude mice, compared to control group (P ＜0.05). Conclusion Artesunate induces a
dose-dependent suppression of BGC-823 tumor growth in nude mice, which might be related to the down-regulation of VEGF and the up-regulation of Calpain2. |
| Key words: Artesunate Stomach neoplasms Nude mice model |
