| 摘要: |
| 目的分析金华地区慢性乙型肝炎患者HBV基因型分布特征及HBV耐药基因位点突变情况，并探讨两者的关系。方法选取104例经核苷（酸）类似物治疗的慢性乙型肝炎患者为研究对象，采用Sanger双脱氧法测序技术检测患者血清HBV的基因型和10种常见的HBVP区耐药基因位点突变情况，并分析两者关系。结果本组慢性乙型肝炎患者中，HBVB基因型占46.15%，C基因型占53.85%，未发现其他基因型。检测出发生耐药基因位点突变61例，突变率为58.65%，其中C基因型占65.57%，B基因型占34.43%。HBVP区耐药基因位点突变率最高的为M204V/I（73.85%），其次为L180M（36.92%）和A181V/T（23.08%）。HBVP区耐药基因发生单位点突变的占50.82%，其中M204I突变类型最多（29.51%）；发生多位点突变的占49.18%；以M204I和M204V/I+L180M两种突变类型最常见。HBVC基因型的患者耐药基因突变率高于B基因型的患者（71.43%vs43.75%，P＜0.05），其中C基因型的患者A181V/T位点突变率高于B基因型的患者（14.29%vs2.08%，P＜0.05）。结论金华地区慢性乙型肝炎患者HBV基因型以B、C基因型为主；HBVP区耐药基因位点突变类型以M204I和M204V/I+L180M两种类型最常见；HBVC基因型的患者比B基因型的患者更易发生耐药突变，且更易发生A181V/T位点突变。 |
| 关键词: 慢性乙型肝炎 耐药突变 基因型 核苷（酸）类似物 |
| DOI：10.12056/j.issn.1006-2785.2017.39.14.2017-905 |
| 分类号: |
| 基金项目: |
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| Genotypes and mutation patterns of HBV gene in chronic hepatitis B patients in Jinhua |
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LIU Ying, ZHU Yijun, WANG Yue
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Jinhua Central Hospital
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| Abstract: |
| Objective To analyze genotypes and mutation patterns of HBV gene in chronic hepatitis B patients in Jinhua. Methods One hundred and four patients with chronic hepatitis B who were treated with nucleoside analogues, were enrolled in the study. Sanger sequence analysis of HBV genotypes and 10 common sites of HBV P region were performed. Results There were 46.15% cases of B genotype and 53.85% of C genotype among 104 chronic hepatitis B patients. Other genotypes were no detected. HBV P gene mutation were detected in 61 cases (58.65%) ; there was 65.57% in HBV C genotype and 34.43% in B genotype. M204V/I was the most common site of HBV P gene mutation (73.85%), followed by L180M (36.92%) and A181V/T (23.08%). 50.82% cases had single site mutation and the most mutation pattern was M204I (29.51%); and 49.18% had multiple sites mutation; the most common mutation patterns were M204I and M204V/I+L180M. The resistance mutation rate in C genotype
was higher than that in B genotype(71.43% vs 43.75%, P＜0.05) and the mutation rate of A181V/T in C genotype cases was high-
er than that in B genotype cases (14.29% vs 2.08%, P＜0.05). Conclusion Genotype B and C are the main genotypes of HBV in this study. The most common mutation patterns of HBV P gene were M204I and M204V/I+L180M. Genotype C patients had more mutations than genotype B and the A181V/T mutation pattern was more common in genotype C. |
| Key words: Chronic hepatitis B Resistance mutation Genotype Nucleoside analogues |
