| 摘要: |
| 目的研究维生素D受体（VDR）基因启动子甲基化异常与婴儿尿路感染的关系。方法选择急性尿路感染患儿32例（尿路感染组）和同期健康体检婴儿30例（健康对照组）。采用荧光定量PCR法检测VDR、DNA甲基化转移酶（DNMTs）mRNA表达水平，重亚硫酸氢钠测序技术检测VDR基因启动子的甲基化水平。结果尿路感染组患儿VDRmRNA表达水平高于健康对照组婴儿，DNMT1mRNA表达水平低于健康对照组婴儿，两组比较差异均有统计学意义（均P＜0.05）。两组DNMT3a、DNMT3bmRNA表达水平比较差异均无统计学意义（均P＞0.05）。尿路感染组患儿VDR基因启动子甲基化水平显著低于健康对照组婴儿，两组比较差异有统计学意义（P＜0.05）。结论尿路感染患儿VDRmRNA表达水平升高，VDR基因启动子的甲基化水平降低，VDR基因甲基化异常与婴儿尿路感染相关。 |
| 关键词: 维生素 D 受体 甲基化 尿路感染 |
| DOI：10.12056/j.issn.1006-2785.2018.40.12.2018-317 |
| 分类号: |
| 基金项目:浙江省医药卫生科技项目（2017186819） |
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| Relationship between abnormal methylation of vitamin D receptor gene promoter and urinary tract infection in infants |
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| Abstract: |
| Objective To analyze the relationship between methylation of vitamin D receptor(VDR) gene promoters and urinary tract infection (UTI) in infants. Methods Thirty two infants aged 1～12 months with UTI (UTI group) and 30 normal infants with matched ages ( control group) were enrolled in the study. The mRNA expressions of VDR and DNA methyltransferase
(DNMT1, DNMT3a, DNMT3b) were detected by real-time PCR; the methylation levels of VDR gene promoters were detected by bisulfite sequencing(BSP). Results The expression of VDR mRNA in UTI group was higher than that in control group(P＜0.05). The value of DNMT1 mRNA in UTI group was lower than that in control group(P＜0.05). There was no significant difference in expression levels of DNMT3a mRNA and DNMT3b mRNA between two groups (both P ＞0.05). The methylation of VDR gene promoter in UTI group was significantly lower than that in control group(P＜0.05). Conclusion The expression level of VDR
mRNA is up-regulated in patients with UTI, while the methylation of VDR gene promoter is decreased, abnormal methylation of VDR gene is associated with urinary tract infection in infants. |
| Key words: Vitamin D receptor Methylation Urinary tract infection |
