| 摘要: |
| 目的探讨冬凌草甲素抑制破骨细胞分化的作用及其机制。方法通过体外培养原代骨髓巨噬细胞（BMMs），观察不同浓度冬凌草甲素对BMMs增殖的抑制作用和破骨细胞分化的影响；抗酒石酸酸性磷酸酶（TRAP）染色观察破骨细胞数目和铺展率；qRT-PCR法检测破骨细胞降钙素受体（CTR）、组织蛋白酶K（CTSK）、活性T细胞核因子c1（NFATc1）mRNA表达水平。结果与对照组相比，经冬凌草甲素处理48、72h后，6.4滋M以上的冬凌草甲素对BMMs的增殖有明显抑制作用，差异均有统计学意义（均P＜0.05）；经冬凌草甲素处理96h后，3.2滋M以上的冬凌草甲素对BMMs的增殖有明显抑制作用，差异均有统计学意义（均P＜0.01）；在NF-资B受体活化因子配体诱导下，与对照组相比，0.4、0.8滋M冬凌草甲素可明显减少破骨细胞分化数目和铺展率（均P＜0.01），且其CTR、CTSK、NFATc1mRNA表达水平均下调（均P＜0.01）。结论冬凌草甲素通过下调破骨细胞分化转录调控因子NFATc1的表达抑制破骨细胞的分化。 |
| 关键词: 冬凌草甲素 破骨细胞 细胞分化 |
| DOI：10.12056/j.issn.1006-2785.2018.40.12.2018-403 |
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| Effect of Oridonin on proliferation and differentiation of osteoclasts and its mechanisms |
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| Abstract: |
| Objective To investigate the effect of oridonin on proliferation and differentiation of osteoclasts and its mechanism. Methods The mouse primary bone marrow-derived macrophages(BMMs) were isolated and treated with 50ng/ml RANKL and 30ng/ml M-CSF to differentiate to osteoclasts. BMMs were treated with different concentration of oridonin and the cell proliferation was determined by CCK-8 assay. Under RANKL stimulation, the number and area of osteoclast were measured by
anti-tartaric acid staining(TRAP); the expression of osteoclast-related genes was detected by qRT-PCR. Results Compared to the control group, after treated by＞6.4滋M oridonin for 48 and 72h, or by＞3.2滋M toridonin for 96h the proliferation of BMMs was significantly inhibited (all P＜0.05 ). Compared to the control group, after treated with 0.4 and 0.8滋M oridonin for 96h, the number and area of osteoclast were significantly reduced( all P＜ 0 . 01 ) , the expressions of CTR , CTSK and NFATc1 mRNA were suppressed (all P＜0.01). Conclusion Oridonin inhibits osteoclast differentiation via suppressing NFATc1 expression. |
| Key words: Oridonin Osteoclasts Cell Differentiation |
