| 摘要: |
| 目的探讨选择性雌激素受体调节剂（SERMs）对小鼠肥胖和脂代谢的作用。方法采用随机数字表法将32只8周龄C57/BL6雌鼠分为假手术组、卵巢切除组、假手术他莫昔芬组、卵巢切除雌激素替代组4组，每组8只。假手术组小鼠腹腔注入0.3%戊巴比妥钠麻醉（0.25ml/10g），打开腹腔暴露卵巢不作处理，随即关闭腹腔；卵巢切除组小鼠腹腔注入0.3%戊巴比妥钠麻醉后行双侧卵巢切除术；假手术他莫昔芬组小鼠麻醉后行假手术，术后每周1次皮下注射他莫昔芬缓释剂0.1ml；卵巢切除雌激素替代组小鼠行双侧卵巢切除术后，皮下埋植17β-雌二醇（E2）缓释剂。予以高脂饲料喂养8周后，比较各组小鼠体重增加量、皮下脂肪/体重比值及内脏脂肪/体重比值、糖耐量、胰岛素抵抗指数（HOMA-IR）、血清E2水平、脂代谢指标TG、TC、HDL-C、LDL-C和血清脂联素（ADPN）水平。结果高脂饲料喂养8周后，假手术他莫昔芬组小鼠体重增加量最少，E2水平明显下降，血清TC、HDL-C水平也最低，差异均有统计学意义（均P＜0.05）；此外，该组小鼠皮下脂肪/体重比值及内脏脂肪/体重比值也显著低于卵巢切除组（均P＜0.05），糖耐量水平明显改善（P＜0.05），血清ADPN水平也明显高于卵巢切除组（P＜0.05），但与卵巢切除雌激素替代组比较差异无统计学意义（P＞0.05）。结论SERMs显著降低血清雌激素水平，对小鼠脂肪蓄积、再分布及血清脂代谢指标有一定的改善作用。 |
| 关键词: 雌激素 选择性雌激素受体调节剂 肥胖 脂代谢 |
| DOI：10.12056/j.issn.1006-2785.2018.40.12.2018-831 |
| 分类号: |
| 基金项目:浙江省自然科学基金青年基金项目（LQ18H040004）；浙江省医药卫生科技计划项目（2016KYA029） |
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| Effects of selective estrogen receptor modulator tamoxifen on obesity and lipid metabolism in mice |
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| Abstract: |
| Objective To investigate the effect of selective estrogen receptor modulator tamoxifen on obesity and lipid metabolism in mice. Methods Thirty two female C57/BL6 mice of 8 weeks were randomly divided into 4 groups: sham group, OVX group, Sham+TAM and OVX +E2 with 8 mice in each group. Sham group:0.3% pentobarbital sodium (0.25ml/10g) was injected into the abdominal cavity, the ovary was exposed before the abdominal cavity was closed. OVX group: bilateral ovariectomy was performed after the mice were anesthetized. Sham+TAM group: sham operation was performed and 0.1ml sustained-release tamoxifen was injected subcutaneously once a week after surgery. OVX +E2 group: bilateral ovariectomy was performed, sustained-release E2 was implanted subcutaneously. After giving high-fat diet for 8 weeks, body weight, subcutaneous and visceral fat weight, glucose tolerance, insulin resistance(HOMA-IR) and serum lipid metabolism related factors were compared among 4 groups. Results The serum estrogen level of Sham+TAM group was decreased, however, its weight
gain was the lowest and the serum TC level was also the lowest (all P＜0.05); in addition, the visceral fat weight of Sham+TAM
group was lower than that of OVX group (P＜0.05), the glucose tolerance was improved(P＜0.05), serum adiponectin (ADPN) level was higher than OVX group (P＜0.05), but there was no significant difference when comparing with OVX+E2 group (P ＞0.05). Conclusion The elective estrogen receptor modulator tamoxifen can significantly reduce the serum estrogen level, at the same time, it also has a positive effect on the body fat accumulation, redistribution and lipid metabolism. |
| Key words: Estrogen Selective estrogen receptor modulators ( SERMs) Obesity Lipid metabolism |
