| 摘要: |
| 目的 探究上皮性卵巢癌组织中纺锤体与着丝粒相关蛋白3(SKA3)的表达情况和意义。方法 选取南京医科大学附属淮安第一医院2014年1月至2018年12月收治的96例上皮性卵巢癌患者视为研究组组,同时期40例卵巢正常患者作为对照组。首先采用实时荧光定量PCR法检测上皮性卵巢癌组织和正常组织中SKA3的mRNA表达水平。接着对两组的组织标本进行免疫组织化学检测,测定其中SKA3蛋白表达量,分析SKA3与上皮性卵巢癌患者的临床病理学特征的相关性。使用Kaplan-Meier生存分析法衡量SKA3的表达水平与上皮性卵巢癌患者预后的关系。最后使用Cox多因素分析患者预后相关的临床病理学特征。采用实时荧光定量PCR和western blot检测人正常卵巢上皮细胞系和上皮性卵巢癌细胞系中SKA3的表达。采用免疫荧光染色检测SKA3在上皮性卵巢癌细胞中的定位。采用一系列功能实验检测SKA3对上皮性卵巢癌细胞增殖、凋亡、迁移、侵袭和上皮间质转化过程的影响。结果 实时荧光定量PCR法的结果表明,SKA3的mRNA水平在上皮性卵巢癌组织中高表达。免疫组织化学染色法显示上皮性卵巢癌组织中SKA3的阳性表达率为84.37%,高于正常卵巢组中的表达水平(χ2=22.104,P＜0.05),且SKA3的高表达与患者病理级别、国际妇产科联盟(FIGO)分期和淋巴结转移相关；生存分析提示高表达的SKA3的上皮性卵巢癌患者具有较差的预后(P=0.002),Cox多因素分析显示SKA3表达水平是上皮性卵巢癌患者预后不良的独立危险因素之一(P=0.004)。实时荧光定量PCR法的结果表明,SKA3在上皮性卵巢癌细胞中表达上调(P＜0.05,P＜0.01和P＜0.001)。免疫荧光染色结果显示,SKA3在上皮性卵巢癌细胞的细胞质中大量表达。功能实验结果显示,敲低SKA3显著抑制上皮性卵巢癌细胞的增殖、迁移、侵袭和上皮间质转化过程,促进细胞凋亡(P＜0.01)。结论 SKA3在上皮性卵巢癌组织和细胞中表达上调,敲低SKA3显著抑制上皮性卵巢癌细胞的增殖、迁移、侵袭和上皮间质转化过程,可以作为上皮性卵巢癌患者的预后生物标志物。 |
| 关键词: 上皮性卵巢癌 免疫组化 纺锤体与着丝粒相关蛋白3 预后 上皮间质转化 |
| DOI： |
| 分类号:R737.31 |
| 基金项目:淮安市基础研究计划（联合专项）卫生健康类科研项目 |
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| Expression of Spindle and kinetochore associated complex subunit 3 in Epithelial Ovarian Cancer and Its Significance in Prognosis |
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Zhang lei1,2,3,4,2,3,5, gongmi6,7,5, Han yaqing6,7,5
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1.Department of gynecology, the Affiliated Huaian NO.1 People&2.amp;3.#39;4.&5.s Hospital of Nanjing Medical University;6.Department of gynecology, the Affiliated Huaian NO.1 People'7.'
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| Abstract: |
| Objective To investigate the expression and significance of Spindle and kinetochore associated complex subunit 3 (SKA3) in epithelial ovarian cancer. Methods A total of 94 patients with pathologically confirmed epithelial ovarian cancer admitted to the Affiliated Huai "an No.1 Hospital of Nanjing Medical University from January 2014 and December 2018 wereselected as the research group, and 40 patients with normal ovaries selected as the control group, Immunohistochemistry was used to detect the expression of SKA3 protein in epithelial ovarian cancer tissues and normal ovarian tissues, and the correlation between SKA3 protein and clinicopathological characteristics of epithelial ovarian cancer patients was evaluated. Cox multivariate analysis and Kaplan-Meier survival analysis were used to estimate the relationship between SKA3 expression and patient survival. Real-time PCR and western blot were used to detect the expression of SKA3 in human normal ovarian epithelial cell lines and epithelial ovarian cancer cell lines. Immunofluorescence staining was used to detect the localization of SKA3 in epithelial ovarian cancer cells. A series of functional experiments were used to detect the effects of SKA3 on the proliferation, apoptosis, migration, invasion and epithelial-mesenchymal transition of epithelial ovarian cancer cells. Results Immunohistochemical staining showed that the SKA3 expression level in ovarian tissue sections was also significantly higher than that in normal ovarian tissues (χ2=22.104, P < 0.05), and the high SKA3 expression was significantly correlated with the pathological grade, the international federation of gynecology and obstetrics (FIGO) stage and lymph node metastasis of the patients. Survival analysis suggested that epithelial ovarian cancer patients with high SKA3 expression had a poor prognosis (P=0.002), and COX multivariate analysis showed that SKA3 expression level was an independent risk factor for poor prognosis of epithelial ovarian cancer patients (P=0.004). The results of real-time fluorescence quantitative PCR showed that the expression of SKA3 was up-regulated in epithelial ovarian cancer cells (P<0.05, P<0.01 and P<0.001). The results of immunofluorescence staining showed that SKA3 was highly expressed in the cytoplasm of epithelial ovarian cancer cells. The results of functional experiments showed that knockdown of SKA3 significantly inhibited the proliferation, migration, invasion and epithelial-mesenchymal transition of epithelial ovarian cancer cells, and promoted cell apoptosis (P <0.01). Conclusion SKA3 expression is up-regulated in epithelial ovarian cancer tissues and cells, and knockdown of SKA3 significantly inhibits the proliferation, migration, invasion and epithelial-mesenchymal transition of epithelial ovarian cancer cells. SKA3 ovarian cancer and may be used as a prognostic biomarker for epithelial ovarian cancer patients. The expression of SKA3 is up-regulated in epithelial ovarian cancer tissues and cells. |
| Key words: : Epithelial ovarian cancer Immunohistochemistry Spindle and kinetochore associated complex subunit 3 Prognosis |