| 摘要: |
| 目的观察不同浓度高糖对小鼠足细胞活性的抑制作用，以及不同浓度雷公藤内酯醇（TP）和缬沙坦（Val）对高糖抑制后小鼠足细胞活性的影响，探讨高糖对足细胞的损伤作用，以及有效的药物干预浓度范围。方法将培养成熟的小鼠足细胞随机分为对照组（11.1mmol/L葡萄糖）和不同浓度高糖组（16.1、21.1、26.1、31.1、36.1mmol/L），以上述浓度培养48h后采用CCK-8检测足细胞活性的变化。取活性变化最大的浓度为高糖诱导浓度，在此基础上随机分为不同浓度的TP组（4、8、16、32、64ng/ml）和Val组（2×10-8、2×10-7、2×10-6、2×10-5、2×10-4mol/L），以上述浓度干预48h后，采用CCK-8检测足细胞活性的变化。结果（1）与对照组相比，除16.1mmol/L高糖组外，其余各高糖组的足细胞活性显著减少，其中以26.1mmol/L葡萄糖组减少最为明显（P＜0.01）。（2）与26.1mmol/L葡萄糖组相比，TP组（除4ng/ml组外）和Val组（除2×10-8mol/L组外）足细胞活性部分恢复，其中以16ng/mlTP组和2×10-5mol/LVal组足细胞活性恢复最为明显（P＜0.01）。结论一定浓度范围的TP和Val可部分恢复受高糖抑制的小鼠足细胞活性。 |
| 关键词: 高糖 雷公藤内酯醇 缬沙坦 足细胞 活性干预 CCK-8 |
| DOI： |
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| 基金项目:浙江省中医药科学研究基金计划A类；杭州市科委医疗卫生及重点专科专病科研攻关专项 |
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| Impact of triptolide and valsartan on inhibition of mouse podocyte activity induced by high glucose |
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Hou Pengchao, Hong Yuzhi, Ye Xun
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Guangxing Hosptial Affiliated to Zhejiang Medicine University
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| Abstract: |
| Objective To investigate the impact of triptolide (TP) and valsartan (Val) on the inhibition of mouse podocyte
activity induced by high glucose. Methods Matured mouse podocytes were cultured and randomly divided into control group (11.1mmol/L glucose) and different high glucose groups (16.1, 21.1, 26.1, 31.1 and 36.1mmol/L glucose, respectively). Changes of podocyte activity were detected by CCK-8 after 48h incubated with different concentrations of glucose, and the appropriate concentration of glucose was screened. Then matured podocyte were randomly divided into different TP groups (4, 8, 16, 32 and 64ng/ml TP, respectively) and Val groups (2×10-8, 2×10-7, 2×10-6, 2×10-5 and 2×10-4mol/L Val, respectively) . The changes of podocyte activity were detected by CCK-8 after 48hrs incubation. Results Compared with control group, the podocyte activity in different high glucose groups were significantly inhibited to varying degrees except that in 16.1mmol/L glucose group. The activity of podocyte was most significantly inhibited in 26.1mmol/L glucose group (P＜0.01). Compared with the 26.1mmol/L high glucose group, the podocyte activity of different TP and Val groups were significantly up-regulated to varying degrees except those of
4ng/ml TP group and 2×10-8mol/L Val group. The activity of podocyte was most significantly up-regulated by the 16ng/ml TP and the 2×10-5mol/L Val, respectively (P＜0.01). Conclusion Both triptolide and valsartan can partially rehabilitate the inhibition of mouse podocyte activity induced by high glucose. |
| Key words: High glucose Triptolide Valsartan Podocyte Activity impact CCK-8 |
