| 摘要: |
| 目的 探讨钙蛋白酶-10 基因（CAPN-10）单核苷酸多态性-19（SNP-19）与多囊卵巢综合征（PCOS）的相关性。
方法 采用聚合酶链反应- 限制性片段长度多态性（PCR-RFLP）技术，对107 例PCOS患者（PCOS 组）及111 例健康体检者（对照组）的CAPN-10 SNP-19 进行分析，并测定其性激素和生化指标。PCOS组再根据BMI与胰岛素抵抗（IR）稳态模型指数（HOMA-IR）分为肥胖组与非肥胖组、IR 组与非IR 组，分析CAPN-10 SNP-19 与其关系。结果 与对照组相比，PCOS 组BMI、空腹血糖（FPG）、TG、TC、LDL、黄体生成素（LH）、黄体生成素/ 卵泡刺激素（LH/FSH）、睾酮（T）均升高（均P＜0.01）。PCOS 组CAPN-10 SNP-19 1等位基因及1/2基因型频率均高于对照组（均P＜0.05）；且CAPN-10 SNP-19 1/2基因型PCOS 发病风险较1/1、2/2 基因型增加约2倍（OR=1.9，95％CI=1.1~3.3，P＜0.05）。CAPN-10 SNP-19 1、2等位基因频率PCOS 肥胖组与非肥胖组、IR 组与非IR组比较均无统
计学差异（均P＞0.05）；PCOS 肥胖组和IR组CAPN-10 SNP-19 1/2基因型频率分别高于非肥胖组和非IR 组，但差异均无统计学意义（均P＞0.05）。结论 CAPN-10 SNP-19 与PCOS 遗传易感性有关，可能与PCOS 肥胖及IR 无关。 |
| 关键词: 多囊卵巢综合征 半胱氨酸内肽酶类，钙蛋白酶-10 多态性，单核苷酸 胰岛素抵抗 |
| DOI： |
| 分类号: |
| 基金项目:浙江省科技计划项目 |
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| Association of CAPN-10 SNP-19 with polycystic ovary syndrome |
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DENG Miao, LIU Yuanwei, ZHANG Hongyan, CEN Jiaping, ZHANG Zhifen
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Hangzhou First People's Hospital
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| Abstract: |
| Objective To investigate the relationship between single nucleotide polymorphisms-19 (SNP-19) in calpain-10 (CAPN-10) gene and polycystic ovary syndrome (PCOS). Methods One hundred and seven PCOS patients(PCOS group) and 111 healthy female subjects(control group) were recruited in the study. The distribution of CAPN-10 SNP-19 was analyzed by polymerase chain reaction- restriction fragment length polymorphism (PCR-RFLP). Serum hormones and biochemical indicators were also measured. Oral glucose tolerance test (OGTT) was performed in PCOS group. Results The BMI, FPG, TG,TC, LDL, LH, LH/FSH and T levels were significantly increased in PCOS group compared to control group(P＜0.01).The frequency
of allele 1 and genotype 1/2 of CAPN-10 SNP-19 were higher in PCOS group than those in control group. Genotype 1/2 was closely associated with PCOS compared to 1/1 and 2/2(OR=1.9, 95％CI=1.1-3.3, P＜0.05). There was no significant difference in frequency of genotype 1/2 between obese group and non-obese group, between insulin resistance (IR) group and non-IR group of PCOS patients (P＞0.05). Conclusion The SNP-19 in CAPN-10 may contribute to the genetic susceptibility to PCOS, but it
may be not correlate with obesity or IR of PCOS patients. |
| Key words: Polycystic ovary syndrome Cysteine endopeptidases,CAPN-10 Polymorphism, single nucleotide Insulin
resistance |
