| 摘要: |
| 目的通过观察银杏叶提取物注射液（Egb761）对抗凝家兔 PLT、PT、APTT、血小板凝血功能的作用，探讨 Egb761 对华法令抗凝的影响。 方法 48 只家兔随机分成两大组，第 1 大组服华法令抗凝，使 PT 达到正常值的 1.5~2.5 倍（国际标准化比值 为 1.8~3.7），第 2 大组服华法令抗凝使 PT 达到正常值的 2.5~3.5 倍（国际标准化比值为 3.7~6.0），每大组又分成对照组（耳缘静脉注 射 0.9%氯化钠注射液 0.3ml/kg），小剂量组（耳缘静脉注射银杏叶提取物注射液 0.3ml/kg），中剂量组（耳缘静脉注射银杏叶提取物注 射液 1.5ml/kg）和大剂量组（耳缘静脉注射银杏叶提取物注射液 3.0ml/kg），每组 6 只（雌雄对半），连续给药 7d，于给药的第 7 天静脉 注射金纳多，1.5h 后自耳缘动脉采血 5ml,进行 PLT、PT、APTT、血小板 3 因子有效性（PF3aT）等检测。 结果 Egb761 对抗凝家兔的 PT、APTT、PLT 均无影响（均 P ＞0.05），但影响血小板 3 因子（PF3），使 PF3aT 的第 3 管较第 4 管延长（P＜0.05），且长 INR 实验组的 影响明显大于短 INR 组。 结论 Egb761 通过影响血小板活化，进而影响 PF3，使机体凝血功能缺陷，并与华法令产生协同作用,使机 体凝血功能缺陷更明显，结果使机体处于易出血状态，从而影响华法令抗凝治疗。 |
| 关键词: 银杏叶提取物注射液 家兔 华法令 抗凝治疗 |
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| Effect of ginkgo bibola leaves extract on coagulation status in rabbits with anticoagulant |
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TANG Zhimiao,NI Yiming
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Jinhua Central Hospital
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| Abstract: |
| Objective To investigate the effect of extract of ginkgo bibola leaves (Egb) on coagulation status in rabbits with anticoagulant. Methods Forty eight rabbits were divided randomly into two groups (n=24 in each group). Warfarin was given in group 1 to achieve 1.8~3.7 times INR of normal rabbits; and given in group 2 to achieve3.7~6.0 times INR. Each group was further divided into 4 subgroups with 6 animals in each, including three Egb groups and one control group. Intravenous infusion of 0.3ml/kg NS was given to the rabbits in control group, and 0.3ml/kg, 1.5ml/kg, 3ml/kg Egb761 were given to the rabbits in three Egb groups for 7 days, respectively. After 1.5 h of injection on d7, the blood samples were collected, and PLT, PT, APTT, PF3aT were examined. Results Egb761 had no effect on PLT, PT, APTT of anticoagulant rabbits(P ＞0.05); while it affected PF3aT levels (P＜0.05), the effect was more marked in group 1 than that in group 2. Conclusion Injection of ginkgo bibola leaves extracts may affect anticoagulation therapy of warfarin in rabbits. |
| Key words: Injection of ginkgo bibola leaves extract Rabbit Warfarin Anticoagulation therapy |
