| 摘要: |
| 目的 通过检测miR-130a 和miR-125b 在伴或不伴冠脉扩张（CAD）川崎病（KD）患儿与正常健康儿童外周血单核细胞（PBMC）中的表达差异，探讨两者可否作为KD 和CAD诊断及预后评估的全新血清生物标志物。方法 利用基因芯片技术筛选出KD 患儿与正常健康儿童之前具有差异表达的miRNAs，通过生物信息学分析，确定候选基因。进一步选取KD 患儿30 例（伴或不伴CAD 各15 例）、正常健康儿童15 例，采用茎环RT-PCR 的方法，验证候选基因miR-130a 和miR-125b 在PBMC 中的表达情况。结果 （1）通过基因芯片技术，分析KD 患儿与正常健康儿童PBMC 中存在明显差异表达的miRNA 共63 条，经过生物信息学分析，确定候选基因；（2）通过茎环RT-PCR 验证发现miR-130a 和miR-125b 在患儿IVIG 治疗前表达明显下调，而IVIG治疗后表达明显上调（P＜0.05）；（3）伴CAD 的KD 患儿miR-130a 及miR-125b 表达较不伴CAD 明显升高，且呈正相关性（R2 =0.734，mir-130a；R2 =0.709，miR-125b）；（4）ROC 曲线分析表明miR-130a（特异度87.5%和敏感度77.5%）和miR-125b（特异度83.3%和敏感度76.6%）对KD 及CAD 的判断有较高的特异度和敏感度。结论 PBMC 中的miR-130a 和miR-125b 参与了KD的发生与发展，可作为KD 及CAD诊断及预后评估的一项全新血清生物标志物，并为KD冠脉扩张的防治提供新的思路。 |
| 关键词: 川崎病 microRNA 冠脉扩张 |
| DOI： |
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| 基金项目:浙江省自然科学基金；浙江省医药卫生科技计划 |
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| Plasma miR-130a and miR-125b as biomarkers of coronary artery dilatation in Kawasaki disease |
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CHU Maoping,ZHOU Aihua,HU Chen,WANG Huiying,PAN Lulu,RONG Xing,WU Rongzhou,ZHAO Xianxian
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the Affiliated Changhai Hospital of Second Military University
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| Abstract: |
| Objective To investigate the expression of microRNAs (miRNAs) in peripheral blood mononuclear cells (PBMCs) and plasma of patients with Kawasaki disease (KD). Methods Fifteen KD pediatric patients with coronary artery dilatation (CAD), 15 KD patients without CAD and 15 healthy children were enrolled in the study. The expression of KD-associated miRNAs in PBMC was screened by microarray. Results The results showed that 63 miRNAs were differentially expressed in PBMCs between the KD group and control group. Two miRNAs miR-130a and miR-125b down-regulated more than 3-fold were selected for study. The expression of miR-130a and miR-125b in plasma samples of KD patients was detected by stem-loop RT-PCR before and after treatment with intravenous immunoglobulin. The expression of miR-130a and miR-125b were higher after treatment than that before treatment (P＜0.05). miR-130a and miR-125b in KD patients were correlated with coronary artery dilatation(CAD) (R2 = 0.734 and R2= 0.709, respectively). Receiver operating characteristic analysis indicated that miR-130a had relatively high sensitivity and specificity to KD with CAD (87.5% and 77.5%, respectively). For miR-125b, these values were 83.3% and 76.6%. Conclusion miR-130a and miR-125b may be associated with the progression of KD and they might be used as potential biomarkers for the diagnosis and prognosis of KD patients with CAD. |
| Key words: Kawasaki disease microRNA Coronary artery dilatation |
