| 摘要: |
| 目的 建立II、IV期胃腺癌及癌旁胃组织的蛋白质表达谱并分析其表达的差异。方法 从胃腺癌II期和IV期患者各3例提取胃癌组织（CaII和CaIV）及胃旁组织（PII和PIV）中总蛋白，采用双向凝胶电泳获得CaII、CaIV、PII、PIV胃组织蛋白质表达谱，通过基质辅助激光解吸电离串联飞行时间质谱（MALDI-TOF/TOF）对主要差异蛋白进行分析。结果PII与CaII之间蛋白质表达有显著差异（达2.0倍以上）的蛋白点有28个，CaII3个蛋白点表达上调，25个蛋白点表达下调；PIV与CaIV之间蛋白质表达有显著差异（达2.0倍以上）的蛋白点有25个，CaIV这些蛋白点表达均下调。使用MALDI－TOF/TOF质谱成功鉴定到8个蛋白点在CaII和CaIV中的表达与PII、PIV均有显著差异，分别是谷胱甘肽巯基转移酶、内质网蛋白ERP29、SH3P、蛋白质膜联蛋白A4、肝脏型脂肪酸结合蛋白、磷酸丙糖异构酶、Transgelin和磷脂酰乙醇结合蛋白，这些蛋白大多与细胞增殖、细胞凋亡及信号转导相关。结论不同分期胃癌与其癌旁组织之间存在蛋白质表达差异，有助于从分子水平了解胃癌的发病机制，探索新的胃癌相关标志物和基因治疗靶点。 |
| 关键词: 胃腺癌 蛋白质组学 双向凝胶电泳 MALDI-TOF/TOF |
| DOI： |
| 分类号: |
| 基金项目:浙江省医药卫生科技计划 |
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| Proteomic analysis of gastric cancer with different stages |
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CHU Meifeng,CHEN Yu,GUO Jianmin,WU Yichun
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Zhejiang Medical College
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| Abstract: |
| Objective To analyze protein profiles in gastric carcinoma with different stages. Methods The total proteins were extracted from specimens of gastric carcinoma TNM stage II and VI (CaII, CaIV) and corresponding pericancerous gastric tissue (PII, PIV). The proteins were separated by two-dimensional gel electrophoresis(2-DE) and analyzed by laser desorption ionization time of flight mass spectrometry(MALDI-TOF/TOF). Results There were 28 significantly spots between PII and CaII (＞2.0), among which 3 spots were up-regulated and 25 spots were down-regulated in CaII; 25 significantly spots were found between PIV and CaIV (＞2.0), all of which were down-regulated in CaIV. Eight spots in gastric carcinoma tissue were identified by MALDI TOF/TOF mass spectrometry. Glutathione S-transferase, ERP29, SH3P, Annexin A4, liver fatty acid binding protein, triosephosphate isomerase isoform 2, transgelin and phosphatidylethanolamine-binding protein 1 were identified in both CaII and CaIV, compared with the corresponding pericancerous tissue. Conclusion Protein expressions are different between gastric cancer tissue and the corresponding pericancerous tissue. |
| Key words: Gastric cancer Proteomics Two-dimensional gel elctrophresis MALDI-TOF/TOF |
