| 摘要: |
| 目的通过培养Hep-2喉癌细胞株，分离并扩增出肿瘤干细胞，放疗后筛选出调控放射抗拒的miRNA。方法含生长因子的无血清培养基培养Hep-2喉癌细胞株，流式细胞仪检测细胞中侧群细胞的比例。当侧群细胞比例达25%时，收集全部细胞球，筛选出CD133+、CD44+细胞作为喉癌干细胞，X线照射喉癌干细胞。miRNA生物芯片检测照射与未照射的喉癌干细胞，筛选出喉癌干细胞中2倍差异表达的miRNA，确定用以调控放射抗拒的miRNA。结果成功培养出侧群细胞，流式细胞仪检测其比例达到26.2%，筛选出的CD133+、CD44+细胞的比例均高于91%。通过miRNA芯片成功检测出喉癌干细胞中2倍差异表达的miRNA。结论本研究成功培养出喉癌干细胞，成功筛选出喉癌干细胞中2倍差异表达的miRNA，发现了可能用以调控放射抗拒的miRNA。 |
| 关键词: 无血清培养基 miRNA 侧群细胞 放疗 |
| DOI： |
| 分类号: |
| 基金项目:2012年杭州市科技计划引导项目 |
|
| Screening of radioresistance-related miRNA from laryngeal cancer stem cells |
|
DUAN Guangliang, DAI Huiping, TANG Tingting, DING Fei, ZHU Guiting
|
|
the Affiliated Hospital of Hangzhou Normal University
|
| Abstract: |
| Objective To screen radioresistance-related miRNA from laryngeal cancer stem cells. Methods Laryngeal cancer Hep-2 cells were cultured in SFM containing growth factor, and cancer stem cells (CD133+ CD44+) were detected and isolated with flow cytometry. The isolated laryngeal cancer stem cells were exposed to high energy X-ray. The differentially expressed miRNA (2-fold) were screened with miRNA biological chips in irradiated and non-irradiated laryngeal cancer stem cells. Results Overtop 91% CD133+ CD44+cells were harvested by FACS. The miRNAs of 2-fold differential expression were successfully screened from irradiated stem cells. Conclusion Laryngeal cancer stem cells have been isolated from Hep-2 cells, and miRNAs related to radioresistance have been screened from laryngeal cancer stem cells. |
| Key words: SFM miRNA Side population Radiotherapy |
