| 摘要: |
| 目的探讨表皮生长因子受体（EGFR）抑制剂对人结肠癌细胞株HT29中CD44+细胞及侧群（SP）细胞的作用机制。方法应用Hoechst33342染色、流式细胞仪检测HT29中CD44+细胞以及SP细胞比例和成瘤性，并对不同浓度EGFR抑制剂吉非替尼作用后球囊形成及SP细胞比例、不同化疗药物处理后CD44+细胞比例进行分析与比较。结果空白对照组HT29中SP细胞比例为（3.82±0.08）%，经维拉帕米阻断后降为（0.34±0.03）%，吉非替尼10、15滋mol/L作用48h后HT29中SP细胞比例明显降低（均P＜0.01），5-氟尿嘧啶（5-FU）作用48h后SP细胞比例变化不大（P＞0.05）。与空白对照组比较，吉非替尼7.5、10、15滋mol/L作用24、48h后CD44+细胞比例均明显降低（均P＜0.05）；且随着吉非替尼的浓度增大，CD44+细胞比例逐渐降低（P＜0.05）。与空白对照组比较，多烯紫杉醇、顺铂作用24、48h后CD44+比例均明显增大（均P＜0.05）。随着时间延长，加入不同浓度的吉非替尼对球囊形成均具有抑制作用。结论EGFR抑制剂对人结肠癌细胞株HT29中CD44+细胞及SP细胞具有生长抑制作用，且可抑制球囊形成。 |
| 关键词: CD44+ 侧群细胞吉非替尼 结肠癌 |
| DOI：10.12056/j.issn.1006-2785.2017.39.01.2015-763 |
| 分类号: |
| 基金项目:上海市自然科学基金（09ZR1418700） |
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| Effect of epidermal growth factor receptor inhibitor on CD44+ cells and side population cells of human colon cancer cell line HT29 |
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GONG Chaojie, TANG Yan, XIA Lu
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Shanghai First People's Hospital,Shanghai Jiaotong University
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| Abstract: |
| Objective To explore the effect of epidermal growth factor receptor (EGFR) inhibitor on CD44+ and side population (SP) cells in colon cancer HT-29 cells. Methods The proportion and tumorigenicity of CD44+ cells and SP cells was detected by Hoechst33342 staining and flow cytometry. The effects of EGFR inhibitor gefitinib at different concentrations on the sphere formation and the proportion of CD44+ cells and SP cells were analyzed. Results EGFR inhibitor significantly inhibited the growth of CD44+ cells and SP cells. The proportion of CD44+ cells in HT29 cells was decreased after treatment with gefitinib for
24h and 48h. The proportion of SP cells in HT29 cells was decreased from (3.82 ±0.08)% to (0.34 ±0.03)% after verapamil
treatment. It was also decreased significantly after gefitinib treatment. Gefitinib had an inhibitory effect on the sphere formation in HT29 cells. Conclusion There are CD44+ and SP cells subpopulation in colon cancer cells. EGFR inhibitor can inhibit the growth of CD44+ and SP cells in colon cancer HT-29 cells, which may provide information for targeting therapy of colon cancer. |
| Key words: CD44+ Side-population cells Gefitinib Colon cancer |
