| 摘要: |
| 目的探讨脂氧素A4（LXA4）、NF-资B在过敏性紫癜（HSP）患儿尿液中的变化及在HSP发病机制中的作用。方法选取50例HSP患儿为病例组，其中紫癜性肾炎（HSPN）患儿20例；同期25例腹股沟疝术前患儿为对照组。留取晨尿，采用ELISA法检测尿液LXA4、NF-资B水平，比较病例组急性期、恢复早期与对照组LXA4、NF-资B水平，并比较HSPN患儿与非HSPN患儿急性期LXA4、NF-资B水平，分析病例组急性期LXA4和NF-资B的相关性。同时收集各组临床资料及病例组24h尿蛋白定量等实验室检查结果，分析HSPN患儿急性期LXA4、NF-资B水平与24h尿蛋白定量的相关性。结果病例组急性期、恢复早期尿液LXA4水平均显著高于对照组（均P＜0.01），且急性期显著低于恢复早期（P＜0.01）；病例组急性期、恢复早期尿液NF-资B水平亦均显著高于对照组（均P＜0.05），且急性期显著高于恢复早期（P＜0.01）。HSPN患儿急性期尿液LXA4水平显著低于非HSPN患儿，NF-资B水平显著高于非HSPN患儿，差异均有统计学意义（均P＜0.05）。病例组急性期尿液LXA4水平与NF-资B水平呈负相关（r=0.347，P＜0.05）。HSPN患儿急性期24h尿蛋白定量与LXA4水平呈负相关（r=0.562，P＜0.05），与NF-资B无相关性（r=0.370，P＞0.05）。结论NF-资B可能参与HSP发病，并与病情严重程度相关，而LXA4可能为肾脏保护因子，且可能通过抑制NF-资B发挥抗炎作用。 |
| 关键词: 过敏性紫癜 儿童 脂氧素 A4 NF-资B 24h 尿蛋白 |
| DOI：10.12056/j.issn.1006-2785.2017.39.21.2017-1597 |
| 分类号: |
| 基金项目:浙江省医药卫生平台研究计划（2015ZDA027）；宁波市医学科技项目（2015C50014）；宁波市自然基金项目（2014A610280） |
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| Urinary lipoxin A4 and NF-资B levels in children with Henoch-Schonlein purpura and their clinical significance |
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College of Medicine,Ningbo University
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| Abstract: |
| Objective To investigate the changes of lipoxin A4 (LXA4) and nuclear factor-资B (NF-资B) levels in the urine of children with Henoch-Schonlein purpura(HSP), and their clinical significance. Methods Fifty children with HSP, including 20 with HSP nephritis (HSPN), and 25 age-matched children with inguinal hernia (control group) were enrolled in the study. The morning urine specimens were collected and ELISA was used to determine the levels of LXA4 and NF-资B. LXA4 and NF-资B levels were compared between case and control groups, and among different subgroups, and the correlation between LXA4 and NF-资B was analyzed. Results The urinary LXA4 levels in acute phase and early recovery phase of HSP patients were significantly higher than
those in control group(both P＜0.01), and the urinary LXA4 levels in acute phase patients were significantly lower than those in early recovery phase (P ＜0.01). The urinary NF-资B levels in acute phase and early recovery phase of HSP patients were also significantly higher than those in control group (both P＜0.05), while the urinary levels of NF-资B in acute phase was significantly higher than those in early recovery phase (P＜0.01). The urinary levels of LXA4 in acute phase of HSP patients without kidney damage were significantly higher than those in HSPN, and the urinary levels of NF-资B were significantly lower (P＜0.05). There was a significantly negative correlation between the urinary levels of LXA4 and NF-资B (r=0.347, P＜0.05) in acute phase HSP patients. Meanwhile, there was a significantly negative correlation between LXA4 and 24-h urinary protein (r=0.562, P＜0.05), while there was no correlation between NF-资B and 24-h urinary protein (r=0.370, P ＞0.05) in acute phase of HSPN. Conclusion NF-资B may be involved in the pathogenesis of HSP, and associated with disease severity. LXA4 may protect kidney against inflammation by inhibiting NF-资B. |
| Key words: Henoch-Schonlein purpura Children Lipoxin A4 Nuclear factor-资B 24-hour urinary protein |
