| 摘要: |
| 目的探讨声触诊组织量化技术（VTQ）和超声微泡造影技术对大鼠肝纤维化的评估价值。方法将84只SPF级雄性Sprague-Dawley大鼠按随机数字表法分为模型组（70只）和对照组（14只）。模型组大鼠采用硫代乙酰胺（TAA）注射法造模，对照组予等量0.9%氯化钠注射液处理。采用超声VTQ检查大鼠肝脏剪切波速度（Vs），采用超声微泡造影定量检测到达时间（AT）、达峰时间（TTP）、峰值强度（PI）、平均通过时间（MTT）、AUC、肝动-静脉渡越时间（HA-HVTT)。结果大鼠肝纤维化程度与Vs呈正相关（r=0.887，P＜0.05），与PI、AUC、HA-HVTT均呈负相关（r=-0.615、-0.782、-0.575，均P＜0.05），即随着肝纤维化程度的加重，出现Vs增加，PI降低，AUC减小，HA-HVTT缩短的趋势。Vs诊断大鼠肝纤维化程度的灵敏度、特异度最高，AUC次之，HA-HVTT与PI相当。结论VTQ和超声微泡造影技术均可有效评估大鼠肝纤维化程度，其在人体的效果值得临床研究。 |
| 关键词: 肝纤维化 声触诊组织量化技术 微泡 诊断 大鼠 |
| DOI：10.12056/j.issn.1006-2785.2017.39.24.2017-1908 |
| 分类号: |
| 基金项目:浙江省自然科学基金资助项目（LY13H180015） |
|
| Assessement of hepatic fibrosis by virtual tough tissues quantification and ultrasound microbubbles in rats |
|
|
|
Hangzhou Xixi Hospital
|
| Abstract: |
| Objective To apply virtual tough tissues quantification (VTQ) and ultrasound microbubbles techniques in evaluation of hepatic fibrosis in rats. Methods Eighty four SPF male Sprague-Dawley rats were randomly divided into model group (n=70) and control group(n=14). Liver fibrosis was induced by injection of thioacetamide(TAA) in model group, while equal volume of normal saline was injected in control group. The liver shear wave velocity (Vs) was examined with VTQ, the time to peak (TTP), peak intensity (PI), mean transit time(MTT), AUC and hepatic artery to hepatic vein transit time (HA-HVTT) were determined with ultrasonic microbubbles. Results The degree of liver fibrosis in rats was positively correlated with Vs (r=0.887, P＜0.05), negatively correlated with PI, AUC and HA-HVTT(r=-0.615, -0.782 and -0.575, all P＜0.05). With the increase of hepatic fibrosis degree, Vs increased and Pi, AUC, HA-HVTT decreased. The sensitivity and specificity of Vs in diagnosis of liver fibrosis degree
in rats were the highest, followed by AUC time, while those of HA-HVTTis and PI were similar. Conclusion VTQ and ultrasound microbubbles might be used in evaluating stages of liver fibrosis clinically and further studies should be conducted. |
| Key words: Liver fibrosis Virtual tough tissues quantification Microbubble Diagnosis Rats |
