| 摘要: |
| 目的探讨叶酸对阿霉素所致心肌损伤的保护作用。方法将24只小鼠随机分成4组：对照组，叶酸组，阿霉素组，阿霉素+叶酸组，每组6只。阿霉素组：采用腹腔内注射阿霉素（每次2.5mg/kg，每周3次，共6次，总量15mg/kg），建立小鼠阿霉素心脏毒性模型，常规喂养4周；叶酸组：以等量0.9%氯化钠溶液代替阿霉素作腹腔内注射，并给予叶酸灌胃[10mg/（kg·d），共4周]；阿霉素+叶酸组：按上两组方法和剂量给予阿霉素和叶酸。对照组：以等量0.9%氯化钠溶液代替阿霉素做腹腔内注射，常规喂养4周。4周后B超测定小鼠心脏功能，TUNEL法测定小鼠心肌细胞凋亡率，并利用Westernblot法分析凋亡相关蛋白Bax、Bcl-2的表达。结果阿霉素组各项心功能指标、心肌细胞凋亡率、Bax/Bcl-2比值与对照组比较均有统计学差异（均P＜0.05）；而阿霉素+叶酸组各项心功能指标、心肌细胞凋亡率、Bax/Bcl-2比值与阿霉素组比较均有明显改善（均P＜0.05）。结论叶酸对小鼠阿霉素心肌损伤有明显的保护作用。 |
| 关键词: 阿霉素 叶酸 心脏毒性 |
| DOI：10.12056/j.issn.1006-2785.2017.39.22.2017-2010 |
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| Folic acid protects against doxorubicin-induced cardiotoxicity |
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the Second Affiliated Hospital of Wenzhou Medical University
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| Abstract: |
| Objective To investigate the protective effects of folic acid(FA) in the pathogenesis of doxorubicin(DOX)- induced cardiotoxicity. Methods Twenty four mice were randomly divided into 4 groups: control group, folic acid group, doxorubicin group and doxorubicin+ folic acid group with 6 mice in each group. Mice in doxorubicin group received doxorubicin (2.5 mg/kg, i.p, 3/wk for 2 wks,); mice in folic acid group received FA 10 mg/d by gavage for 4 wks; mice in doxorubicin+folic acid group received FA10 mg/d by gavage from the day of DOX administration for 4 wks; mice in control group received injections of saline. After 4 weeks, left ventricular (LV) function was measured by echocardiography, cardiomyocyte apoptosis were assessed by TUNEL staining; the expression of bax/bcl-2 was detected by Western blot. Results Doxorubicin induced left ventricular
dysfunction and remolding (P＜0.05), increased cardiomyocyte apoptosis (P＜0.05), and up-regulated of bax/bcl-2 levels (P＜
0.05). FA ameliorated DOX-induced left ventricular dysfunction and remolding(P＜0.05), attenuated cardiomyocyte apoptosis(P＜ 0.05), and down-regulated bax/bcl-2 expression(P＜0.05). Conclusion Folic acid has a protective effect against DOX-induced cardiotoxicity in mice. |
| Key words: doxorubicin ( DOX) folic acid( FA) cardiotoxicity |
