| 摘要: |
| 目的探讨结直肠癌组织中miR-21表达与临床病理因素及预后的关系。方法取59例结直肠癌组织标本及癌旁正常黏膜组织标本，采用茎环逆转录实时荧光定量PCR方法检测结直肠癌组织及癌旁正常黏膜组织中miR-21的相对表达量，并分析癌组织中miR-21表达与临床病理因素的关系。用Kaplan-meier法计算生存率，采用log-rank检验、Cox比例风险回归模型分析结直肠癌预后影响因素。结果结直肠癌组织中miR-21的相对表达量明显高于其癌旁正常黏膜组织（P＜0.05）。结直肠癌组织miR-21表达水平与肿瘤直径、淋巴结转移、临床分期有关（均P＜0.05）；与性别、远处转移、分化程度、浸润深度和肿瘤位置无关（均P＞0.05）。远处转移、分化程度、miR-21表达水平是影响患者预后的独立危险因素（均P＜0.05），其RR值及95%CI分别为5.053（1.395~5.787）、3.998（1.395~18.305）、3.837（1.266~11.631）。结论miR-21与结直肠癌患者预后和疾病进展密切相关。 |
| 关键词: 结直肠癌 miR-21 预后 Cox 比例风险回归模型 |
| DOI：10.12056/j.issn.1006-2785.2017.39.18.2017-41 |
| 分类号: |
| 基金项目:浙江省自然基金项目（Y2100917） |
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| Expression of miR-21 in colorectal cancer and its clinicopathologic significance |
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the First Affiliated Hospital of Wenzhou Medical University
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| Abstract: |
| Objective To investigate the expression of miR-21 in colorectal cancer and its association with clinicopathological characteristics and the prognosis of patients. Methods The expression of miR-21 in 59 specimens of colorectal cancer and matched non-tumor adjacent tissue specimens was examined with stem-loop real-time RT-PCR. The relationship between the expression of miR-21 and clinicopathologic features of colorectal carcinoma was analyzed. Kaplan-Meier survival curves, log-rank test and multivariate Cox regression were used to analyze the factors related to prognosis of colorectal cancer. Results The expression level of miR-21 was significantly higher in colorectal cancer tissue than that in the
matched normal colorectal mucosa (2.698±1.973 vs. 0.653±0.213, P＜0.05). The expression of miR-21 was positively correlated
with tumor size, lymph node metastasis and clinical stage (P＜0.05), but not with sex, distant metastasis, cell differentiation, depth of tumor infiltration and location (P ＞0.05). Kaplan-Meier univariate analysis showed that distant metastasis, cell differentiation, depth of tumor infiltration and miR-21 was the prognostic factor for colorectal cancer (P ＜0.05). The Cox regression analysis showed that distant metastasis, cell differentiation and miR-21 was an independent prognostic risk factor of colorectal cancer(P＜
0.05), RR value and 95%CI was 5.053 (1.395-5.787), 3.998 (1.395-18.305) and 3.837 (1.266-11.631). Conclusion The results indicate that miR-21 serves as a molecular marker for disease progression and prognosis of colorectal cancer. |
| Key words: Colorectal cancer miR-21 Prognosis Cox model |
