| 摘要: |
| 目的探讨microRNA-26a在原发性肝癌（HCC）患者中的表达及临床意义。方法选取HCC、慢性乙型肝炎（CH）患者各28例。HCC患者留取癌组织、癌旁组织、血浆标本，CH患者留取血浆标本，采用RT-PCR法检测标本microRNA-26a的表达水平。比较HCC患者与CH患者microRNA-26a表达水平；分析HCC患者癌组织、癌旁组织microRNA-26a表达水平的相关性；比较血浆microRNA-26a与血清甲胎蛋白（AFP）诊断HCC的灵敏度、特异度；以血浆microRNA-26a水平最佳临界值（2.873）为界，将HCC患者分为microRNA-26a高表达组和低表达组，比较两组患者术后1年生存率。结果HCC患者血浆microRNA-26a表达水平低于CH患者（P＜0.05）。HCC患者癌组织microRNA-26a表达水平低于癌旁组织（P＜0.05）。HCC患者癌组织、癌旁组织microRNA-26a表达水平呈正相关（r=0.574，P＜0.038）。血浆microRNA-26a诊断HCC的灵敏度较血清AFP强（85.8%vs57.8%，P＜0.05），但特异度较血清AFP弱（65.2%vs80.52%，P＜0.05）。microRNA-26a高表达组患者术后1年生存率高于低表达组（60.25%vs45.77%，P＜0.05）。结论HCC患者microRNA-26a表达水平较低，microRNA-26a可作为早期诊断HCC的生物标志物，也可作为HCC患者预后评估指标。 |
| 关键词: 原发性肝癌 microRNA-26a 甲胎蛋白 相关性 生存曲线 |
| DOI：10.12056/j.issn.1006-2785.2017.39.24.2017-542 |
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| Expression of microRNA-26a in primary liver cancer and its clinical significance |
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Pizhou People's Hospital of Jiangsu Province
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| Abstract: |
| Objective To investigate the expression of microRNA-26a (mRNA-26a) in primary hepatocellular carcinoma (HCC) and its clinical significance. Methods The expression of mRNA-26a were detected with RT-PCR in plasma, cancer tissue and pericancerous liver tissue samples from 28 patients with HCC and plasma samples from 28 patients with chronic hepatitis B (CHB). The clinical significance of mRNA-26a expression in HCC was analyzed. Results The expression level of plasma microRNA-26a in HCC patients was lower than that in CHB patients (P＜0.05). The expression level of microRNA-26a in cancer tissues of HCC patients was lower than that of pericancerous liver tissues (P＜0.05). There was a positive correlation between the expression of microRNA-26a in cancer tissues and pericancerous liver tissues (r=0.574, P＜0.05). The sensitivity of plasma microRNA-26a in diagnosis of HCC was higher than that in serum AFP (85.8% vs 57.8%, P＜0.05), but the specificity was
lower than that of serum AFP (65.2% vs 80.52%, P＜0.05). The 1-year survival rate of patients with high expression of mRNA-26a was higher than that of low expression group (60.25% vs 45.77%, P＜0.05). Conclusion The expression level of mRNA-26a in HCC tissues is down-regulated, it may be used as a molecular marker for early diagnosis of HCC. |
| Key words: Primary hepatocellular carcinoma MicroRNA-26a Alpha-fetoprotein Correlation Survival curve |
