| 摘要: |
| 目的探讨IL-6/sIL-6R复合物对人脐静脉内皮细胞（HUVECs）血管内皮生成因子（VEGF）及基质金属蛋白酶-9（MMP-9）表达的影响。方法采用不同浓度的IL-6（0、10、20、40、80ng/ml）联合sIL-6R（100ng/ml）刺激HUVECs，培养24h后，分别采用real-timePCR及Westernblot检测MMP-9、VEGF的mRNA及蛋白表达。结果在基因水平IL-6/sIL-6R复合物可诱导内皮细胞VEGF及MMP-9mRNA的表达；在蛋白水平IL-6/sIL-6R复合物亦可促进VEGF及MMP-9的表达。结论IL-6/sIL-6R复合物上调血管内皮细胞VEGF及MMP-9的表达，在类风湿关节炎（RA）的发病机制中起着重要的作用。 |
| 关键词: 白介素 -6 可溶性白介素 -6 受体 类风湿关节炎 基质金属蛋白酶 -9 血管内皮生长因子 |
| DOI：10.12056/j.issn.1006-2785.2017.40.1.2017-543 |
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| IL-6/sIL-6R complex up-regulates VEGF and MMP-9 expression in human umbilical vein endothelial cells |
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Zhejiang Hospital of Traditional Chinese Medicine
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| Abstract: |
| Objective To investigate the regulatory effect of IL-6/sIL-6R complex on expression of MMP-9 and VEGF in human umbilical vein endothelial cells (HUVECs). Methods Cultured HUVECs were treated with sIL-6R (100ng/ml) and various concentrations of IL-6 (0, 10, 20, 40 and 80ng/ml) for 24h, the expression of MMP-9 and VEGF was detected by real-time fluorescent quantitative PCR and Western blot. Results The mRNA and protein expressions of MMP-9 and VEGF were increased in presence of IL-6/sIL-6R complex (P ＜0.05). Conclusion IL-6/sIL-6R complex can promote the expression of MMP-9 and VEGF in human umbilical vein endothelial cells, indicating that IL-6/sIL-6R complex may be involved in the pathogenesis of rheumatoid arthritis. |
| Key words: IL-6 sIL-6R Rheumatoid arthritis MMP-9 VEGF |
