| 摘要: |
| 目的研究复发性自然流产（RSA）中子宫蜕膜组织前列腺素E2（PGE2）的水平变化及子宫平滑肌细胞前列腺素E受体（EP）的表达分布，探讨PGE2/EP系统在RSA发病中的作用及机制。方法选择37例RSA患者作为RSA组和48例人工流产孕妇作为对照组。采用RT-PCR法检测蜕膜组织中环氧化酶-2（COX-2）、膜相关前列腺素E合成酶（mPGES）和子宫平滑肌细胞中EP表达水平，ELISA法检测蜕膜组织中PGE2和子宫平滑肌细胞中环磷酸腺苷（cAMP）表达水平，利用Fluo-3AM绿色荧光染料检测子宫平滑肌细胞中钙离子浓度。结果RSA组蜕膜组织中COX-2、mPGES和PGE2表达水平均高于对照组，差异均有统计学意义（均P＜0.05）。RSA组子宫平滑肌细胞中EP1和EP3表达水平均高于对照组，而EP2、cAMP表达水平均低于对照组，差异均有统计学意义（均P＜0.05）。RSA组子宫平滑肌细胞中钙离子浓度显著高于对照组，差异有统计学意义（P＜0.01）。结论子宫蜕膜组织PGE2的合成增加和平滑肌细胞EP受体分布异常可促使子宫的异常收缩而导致RSA。 |
| 关键词: 复发性自然流产 子宫平滑肌细胞 前列腺素 E2 前列腺素 E2 受体 |
| DOI：10.12056/j.issn.1006-2785.2018.40.7.2017-716 |
| 分类号: |
| 基金项目:浙江省自然科学基金项目（LY12H04003） |
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| Relationship between the PGE2 receptor distribution in uterine smooth muscle and the pathogenesis of recurrent spontaneous abortion |
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Shool of Medicine,Jinhua Polytechnic
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| Abstract: |
| Objective To investigate the relationship between the distribution of prostaglandin E receptor (EP) in uterine smooth muscle cells and the pathogenesis of recurrent spontaneous abortion (RSA). Methods Thirty seven patients with RSA (RSA group) and 48 women with induced abortion (control group) were enrolled. Expression levels of cyclooxygenase-2 (COX-2) and membrane associated prostaglandin E synthase (mPGES) in decidual tissue, and EP in uterine smooth muscle cells were analyzed by RT-PCR. Prostaglandin E2 (PGE2) in uterine decidua tissue and cyclic adenosine monophosphate (cAMP) in uterine smooth muscle cells were detected by ELISA. Intracellular calcium ion was labeled with Fluo-3AM and images were acquired with Confocal microscope. Results The expressions of COX-2, mPGES and PGE2 in the decidua tissue of RSA group were all higher
than those of control group (all P＜ 0.05). The expression levels of EP1 and EP3 in uterine smooth muscle cells of group RSA were
all higher than those of control group, while EP2 and cAMP were all lower (all P＜ 0.05). The calcium ion concentration in the RSA group was significantly higher than that in the control group (P＜ 0.01). Conclusion The increase of PGE2 synthesis in the uterine decidua tissue and the abnormal distribution of EP receptor in uterine smooth muscle cells may induce abnormal contraction of the
uterus and lead to RSA. |
| Key words: Recurrent spontaneous abortion Smooth muscle Prostaglandin E2 Prostaglandin E2 receptor |
