| 摘要: |
| 目的检测鲍温样丘疹病（BP）皮损中朗格汉斯细胞（LC）的分布与数量变化及单核细胞趋化蛋白-1（MCP-1）的表达，探讨它们在BP发病机制中的作用。方法采用免疫组化SP法检测36例BP患者皮损组织中CD1a+LC及MCP-1的表达情况，以皮损旁正常皮肤组织作为对照；并对CD1a+LC密度与MCP-1表达强度进行相关性分析。结果CD1a+LC主要见于表皮棘层的树突状细胞，MCP-1蛋白主要表达于表皮角质形成细胞。与皮损旁正常皮肤组织相比，BP皮损组织中CD1a+LC密度降低，分布不规则，胞体树突减少；MCP-1染色变弱，表达强度降低。BP皮损组织及皮损旁正常皮肤组织中CD1a+LC密度与MCP-1表达强度均呈正相关（r=0.372、0.384，均P＜0.05）。结论BP皮损表皮组织中LC密度下降，形态呈现变性改变，推测可能与皮损局部趋化LC并促进其分化成熟的趋化因子MCP-1表达降低有关，进而影响皮损局部LC的抗原递呈功能。 |
| 关键词: 鲍温样丘疹病 朗格汉斯细胞 单核细胞趋化蛋白 -1 免疫组化 |
| DOI：10.12056/j.issn.1006-2785.2018.40.9.2018-460 |
| 分类号: |
| 基金项目:浙江省教育厅科研计划项目（Y201327164）；国家自然青年科学基金项目（81301375） |
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| Distribution of Langerhans cells and expression of MCP-1 in skin lesions of Bowenoid papulosis and their clinical significance |
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Hangzhou Medical College
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| Abstract: |
| Objective To investigate the distribution of Langerhans cells (LCs) and the expression of monocyte chemoattractant protein-1 (MCP-1) in skin lesions of Bowenoid papulosis (BP) and their clinical significance. Methods The expression of CD1a (marker of LC) and MCP-1 in skin lesions and adjacent normal skin tissue of 36 patients with BP were detected with immunohistochemical staining. The correlation between the density of CD1a+ LCs and MCP-1 expression was analyzed. Results CD1a+ LCs was mainly expressed in dendritic cells in the spinous layer of the epidermis. MCP-1 was mainly expressed in the cytoplasm of epidermal keratinocytes. In epidermal BP lesions, CD1a+ LCs density was lower, and these cells exhibited an abnormal distribution and shorter protrusions, and MCP-1 staining was weaker and the semi-quantitative score of MCP-1 expression was lower than those in the adjacent normal epidermal tissue. The density of CD1a+ LCs was positively correlated with MCP-1 expression in BP lesions and the adjacent normal skin tissue. Conclusion The decreased LC density and degenerated LC morphology in the epidermis may be related to the down-regulation of MCP-1 expression in BP lesions, ultimately leading to immune suppression. |
| Key words: Bowenoid papulosis Langerhans cells MCP-1 Immunohistochemistry |
